CCNH Antibody

Cyclin-H, Mouse Anti Human
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Cat. No.
BT14671
Synonyms
CCNH, CAK, p34, p37, Cyclin-H, MO15-associated protein.
Appearance
Sterile filtered colorless solution.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Key Features of CCNH Antibodies

ParameterDetails
TypesMonoclonal (Mouse IgG2b) or Polyclonal (Rabbit IgG)
ImmunogensRecombinant human CCNH (amino acids 1–323 or full-length protein)
PurificationProtein-A affinity chromatography or peptide affinity purification
ApplicationsWestern blot (WB), immunohistochemistry (IHC), immunofluorescence (IF)
ReactivityHuman, mouse, rat, porcine, or primate tissues

Cell Cycle Regulation and Kinase Activity

CCNH antibodies are pivotal in studying Cyclin H’s role in:

  • CDK7 activation: Phosphorylates CDK2 and CDC2 to drive cell cycle progression .

  • Transcriptional regulation: Interacts with TFIIH and RNA polymerase II .

Cancer-Related Research

CCNH antibodies enable investigations into oncogenic pathways:

  • CtBP2 interaction: CCNH/CDK7 stabilizes CtBP2, enhancing tumor cell migration and invasion . Depleting CCNH reduces CtBP2 dimerization and repressive activity, impairing metastasis .

  • PD-L1 upregulation: Lnc-CCNH-8, a non-coding RNA, sponges miR-217 to increase PD-L1 expression, promoting immune evasion in hepatocellular carcinoma (HCC). CCNH antibodies may aid in studying this mechanism .

Interaction with CtBP2 in Cancer Metastasis

ExperimentFindingSource
CoimmunoprecipitationEndogenous CCNH binds CtBP2 in MDA-MB-231 cells, indicating a natural interaction .
shRNA knockdownCCNH or CDK7 depletion reduces CtBP2 protein levels and dimerization .
Transwell/Wound assaysCCNH overexpression enhances breast cancer cell invasion and migration .

Lnc-CCNH-8 and PD-L1 Regulation

MechanismImpactSource
miR-217 spongingLnc-CCNH-8 upregulates PD-L1, suppressing CD8+ T-cell cytotoxicity .
PKP3/miR-3173 axisStabilizes PD-L1 protein, enhancing immune evasion .
Plasma exosomal markerHigh Lnc-CCNH-8 correlates with improved response to anti-PD-1/PD-L1 therapy in HCC .

Clinical and Therapeutic Implications

  • Cancer immunotherapy: CCNH antibodies may help stratify HCC patients for PD-1/PD-L1 inhibitors .

  • Targeted therapies: Inhibiting CCNH/CDK7 could disrupt CtBP2-mediated metastasis .

  • Biomarker development: Exosomal Lnc-CCNH-8 serves as a predictive marker for immunotherapy response .

Validation and Quality Assurance

  • Specificity: Antibodies must be validated using knockout (KO) cell lines or orthogonal methods (e.g., mass spectrometry) .

  • Reproducibility: Recombinant antibodies outperform polyclonal ones in consistency .

Product Specs

Introduction
CCNH, a member of the cyclin family, is known for its fluctuating protein levels throughout the cell cycle. Cyclins regulate CDK kinases. CCNH combines with CDK7 kinase and ring finger protein MAT1 to form a complex. This kinase complex phosphorylates CDK2 and CDC2 kinases, acting as a CDK-activating kinase (CAK). Both CCNH and its kinase partner are integral components of TFIIH and RNA polymerase II protein complexes.
Physical Appearance
A clear, sterile-filtered solution.
Formulation
The solution contains 1mg/ml of CCNH antibody in a buffer of PBS at pH 7.4, with 10% glycerol and 0.02% sodium azide.
Storage Procedures
Store at 4°C for up to 1 month. For longer storage, keep at -20°C. Avoid repeated freezing and thawing.
Stability / Shelf Life
The product is stable for 12 months when stored at -20°C and for 1 month at 4°C.
Applications
The CCNH antibody has been validated for specificity and reactivity through ELISA and Western blot analysis. Optimal working dilutions should be determined for each application. A starting dilution of 1:1000 is recommended for Western blot analysis.
Synonyms
CCNH, CAK, p34, p37, Cyclin-H, MO15-associated protein.
Purification Method
CCNH antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
PAT3G6AT.
Immunogen
Anti-human CCNH mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human CCNH amino acids 1-323 purified from E. coli.
Ig Subclass
Mouse IgG2b heavy chain and k light chain.

Product Science Overview

Role in Cell Cycle Regulation

The CAK complex regulates cell cycle progression by activating cdc2, CDK2, and CDK4 kinases through phosphorylation of a critical threonine residue in the T-loop of the CDK-cyclin complexes . The CAK complex can exist either in its free form or in association with transcription factor IIH (TFIIH), which can affect its substrate specificity . When bound to TFIIH, CAK preferentially phosphorylates the carboxy-terminal domain of RNA polymerase II, linking cell cycle control, transcriptional regulation, and DNA repair .

Mouse Anti Human Cyclin-H Antibody

The Mouse Anti Human Cyclin-H Antibody is a polyclonal antibody produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy-terminus of cyclin H . These antibodies are purified by protein A and peptide affinity chromatography . The antibody detects endogenous levels of cyclin H and does not cross-react with other family members at physiological levels .

Applications

This antibody is validated for use in various applications, including:

  • Western Blotting (WB): Used to detect cyclin H in cell extracts.
  • Immunoprecipitation (IP): Used to isolate cyclin H from cell lysates .
Storage and Handling

The antibody is supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, and 50% glycerol. It should be stored at –20°C and should not be aliquoted .

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Related Products

CCNH Human
Cyclin-H Human Recombinant
Cat. No.
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Source
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THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
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