zgc:153146 Antibody

What is zgc:153146 Antibody and what is its target protein?

zgc:153146 Antibody (product code CSB-PA603902XA01DIL) is a polyclonal antibody that targets the protein encoded by the gene initially designated as zgc:153146, now identified by the gene symbol cfap96 (Entrez Gene ID: 751702). This antibody specifically recognizes the cfap96 protein in Danio rerio (zebrafish) . The target protein is cataloged in UniProt with the accession number Q0P4C5 . The antibody is raised in rabbits using recombinant Danio rerio zgc:153146 protein as the immunogen .

What experimental applications is zgc:153146 Antibody validated for?

The zgc:153146 Antibody has been validated for two primary applications: Enzyme-Linked Immunosorbent Assay (ELISA) and Western Blotting (WB) . These techniques allow researchers to detect and quantify the cfap96 protein in zebrafish samples. The antibody's specific reactivity to zebrafish makes it particularly valuable for developmental biology and comparative genomics research using this model organism .

What are the proper storage and handling conditions for zgc:153146 Antibody?

For optimal performance and stability, zgc:153146 Antibody should be stored at either -20°C or -80°C . Proper aliquoting upon receipt is recommended to minimize freeze-thaw cycles. When working with the antibody, standard antibody handling protocols should be followed, including using sterile techniques and maintaining cold chain during experimental procedures. The antibody components include 0.2mg of purified antibody along with positive and negative controls for experimental validation .

What controls are provided with the zgc:153146 Antibody?

The antibody product includes three components designed to facilitate experimental validation:

• 200μg of antigens that serve as a positive control

• 1ml of pre-immune serum that functions as a negative control

• Rabbit polyclonal antibodies purified by Antigen Affinity

These controls are essential for establishing experimental baselines and validating assay specificity when working with this antibody.

How can zgc:153146 Antibody be used in developmental studies of zebrafish?

For developmental biology research, zgc:153146 Antibody can be employed to track expression patterns of the cfap96 protein across different embryonic and larval stages of zebrafish. Researchers should consider the following methodological approach:

• Sample collection at defined developmental timepoints (e.g., hours post fertilization)

• Tissue preparation through either whole-mount protocols or sectioning

• Immunohistochemistry or immunofluorescence optimization using the antibody at different dilutions

• Co-localization studies with known developmental markers

The antibody's specificity to zebrafish makes it particularly valuable for studying the role of cfap96 in organ and tissue development, especially in comparative studies with other model organisms .

What considerations should be made when using zgc:153146 Antibody in co-immunoprecipitation experiments?

When designing co-immunoprecipitation (Co-IP) experiments with zgc:153146 Antibody, researchers should consider:

• Optimization of lysis buffer composition to preserve protein-protein interactions while efficiently extracting the target protein

• Pre-clearing lysates with the provided pre-immune serum to reduce non-specific binding

• Titration of antibody amounts for optimal immunoprecipitation efficiency

• Validation of results using the provided antigen as a positive control

• Selection of appropriate beads (Protein A/G) based on the rabbit IgG isotype of this antibody

The polyclonal nature of this antibody may provide advantages in Co-IP by recognizing multiple epitopes, potentially improving capture efficiency of native protein complexes.

How can epitope prediction improve experimental design with zgc:153146 Antibody?

Advanced researchers can enhance experimental outcomes by applying antibody structure prediction principles to zgc:153146 Antibody applications. Recent advancements in computational methods have improved antibody loop structure prediction accuracy, which is critical for understanding epitope recognition . Researchers should:

• Use available prediction tools to identify likely binding regions of the antibody to the cfap96 protein

• Design experiments that account for potential structural changes in the target protein under different experimental conditions

• Consider how experimental treatments might affect epitope accessibility

• Implement epitope mapping strategies to confirm computational predictions

This approach aligns with modern antibody research methodologies where structural considerations significantly impact experimental outcomes and interpretation .

What strategies can be employed to validate specificity of zgc:153146 Antibody in complex zebrafish tissue samples?

For complex tissue samples, validation of antibody specificity is crucial. Researchers should implement a multi-faceted validation approach:

• Perform parallel experiments using the provided negative control (pre-immune serum)

• Conduct peptide competition assays using the provided antigen

• Include knockout/knockdown samples as negative controls where possible

• Compare staining patterns with mRNA expression data from in situ hybridization

• Validate across multiple detection techniques (WB, ELISA, IHC) to confirm consistent target recognition

These validation steps are particularly important when studying tissues with potential cross-reactive proteins or high background.

What are the optimal dilution ratios for zgc:153146 Antibody in different applications?

Based on available data and standard protocols for polyclonal antibodies, the following dilution ranges are recommended as starting points for optimization:

Each application requires specific optimization based on sample type, detection system, and experimental conditions .

How can researchers troubleshoot non-specific binding when using zgc:153146 Antibody?

When encountering non-specific binding, researchers should implement the following troubleshooting approach:

• Increase blocking stringency using different blocking agents (BSA, normal serum, casein)

• Optimize antibody concentration through careful titration experiments

• Adjust incubation conditions (time, temperature, buffer composition)

• Include additional washing steps with increased salt concentration or detergent

• Utilize the provided pre-immune serum to identify and control for non-specific binding patterns

The polyclonal nature of this antibody means that some degree of background may be present, requiring careful optimization for each specific experimental context.

What considerations should be made when designing quantitative experiments with zgc:153146 Antibody?

For quantitative applications such as protein expression analysis, researchers should implement:

• Standard curve generation using the provided antigen at known concentrations

• Selection of appropriate housekeeping proteins as internal controls

• Technical replicates (minimum of three) to assess assay variability

• Biological replicates to account for natural variation

• Image analysis protocols with defined thresholds for signal quantification

For Western blot densitometry or ELISA absorbance readings, careful normalization procedures should be established to ensure reliable quantitative comparisons across samples .

How can zgc:153146 Antibody be integrated into multi-omics research approaches?

In the context of integrative studies, zgc:153146 Antibody can serve as a valuable tool when combined with other research methodologies:

• Correlation of protein detection (via this antibody) with transcriptomics data on cfap96 expression

• Integration with proteomics datasets to confirm mass spectrometry identification

• Combination with genetic manipulation (CRISPR, morpholinos) to study functional consequences

• Application in ChIP-based approaches if studying protein-DNA interactions

This multi-platform integration provides more comprehensive insights into cfap96 function in zebrafish biology and development .

What considerations should be made when using zgc:153146 Antibody in genetic models with altered cfap96 expression?

When applying this antibody in genetic models (knockdown, knockout, or overexpression):

• Validate antibody specificity in the modified genetic background

• Consider epitope availability in fusion proteins or truncated variants

• Adjust detection protocols based on anticipated expression levels

• Use the antibody to confirm genetic manipulation at the protein level

• Compare experimental results with computational predictions of protein structure and antibody binding

These considerations are particularly important when the antibody is used to validate genetic modifications or to study the consequences of genetic manipulation on protein expression and localization .

How does the specificity of zgc:153146 Antibody compare to other tools for studying cfap96?

The zgc:153146/cfap96 Antibody offers several advantages as a research tool:

• Direct detection of the protein product rather than inference from mRNA

• Species specificity for zebrafish models

• Validation in multiple applications (ELISA, WB)

• Availability of control materials for experimental validation

What future research directions might benefit from zgc:153146 Antibody applications?

Emerging research areas that could leverage this antibody include:

• Structural biology studies of cfap96 protein

• Developmental regulation of cfap96 expression

• Comparative studies across zebrafish strains or related species

• Integration with advanced imaging techniques for protein localization

• Application in protein-protein interaction networks relevant to cfap96 function