CD164 Human
Description
Biological Functions of CD164
Hematopoietic Regulation
CD164 serves as a key regulator of hematopoietic stem/progenitor cells (HSPCs):
Tumorigenic Properties
CD164 overexpression correlates with malignant progression in multiple cancers:
Clinical Significance in Human Cancers
Expression Patterns
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Glioma: 3.8-fold higher mRNA expression vs. normal brain tissue (P<0.001)
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Lung Cancer: 72% of NSCLC cases show strong CD164 staining vs. 22% in normal lung
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Bladder Cancer: High CD164 correlates with distant metastasis (P=0.001)
Prognostic Value
Mechanistic Insights
Stem Cell Maintenance
CD164 overexpression induces:
Signaling Pathways
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CXCL12/CXCR4 axis activation (2.7-fold increase in phosphorylated ERK)
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PTEN suppression leading to PI3K/AKT hyperactivation (85% reduction in PTEN expression)
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mTOR-dependent metabolic reprogramming (40% growth reduction with rapamycin)
Research Applications
Diagnostic Potential
Therapeutic Targeting
Preclinical studies demonstrate:
Product Specs
CD164, also referred to as Multi-Glycosylated Core Protein 24, belongs to the CD164 family of proteins.
CD164, produced using Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain with a molecular weight of 15.6kDa. It consists of amino acids 24-162 and is fused to a 6 amino acid His Tag at the C-terminus, resulting in a total of 148 amino acids. CD164 exhibits multiple bands between 28-57kDa on SDS-PAGE under reducing conditions and is purified using proprietary chromatographic techniques.
The CD164 protein solution has a concentration of 0.5mg/ml and is supplied in a buffer containing 10% glycerol and Phosphate buffered saline (pH 7.4).
The purity of the product is determined by SDS-PAGE analysis and is greater than 85.0%.
CD164 Molecule, Multi-Glycosylated Core Protein 24, CD164 Antigen, Sialomucin, Endolyn MGC-24v, MGC-24, MUC-24, Deafness, Autosomal Dominant 66, Sialomucin Core Protein 24, DFNA66, CD164.
ADPDKNTTQH PNVTTLAPIS NVTSAPVTSL PLVTTPAPET CEGRNSCVSC FNVSVVNTTC FWIECKDESY CSHNSTVSDC QVGNTTDFCS VSTATPVPTA NSTAKPTVQP SPSTTSKTVT TSGTTNNTVT PTSQPVRKST FDHHHHHH
Product Science Overview
CD164 is an 80- to 90-kDa molecule expressed by human CD34+ hematopoietic progenitor cells . It has several isoforms, each with distinct expression patterns and functions:
- Isoform 1: Expressed in hematopoietic and non-hematopoietic tissues, including prostate cancer tumors and cell lines. Its expression is higher in bone metastases compared to primary tumors .
- Isoform 2: Found in the small intestine, colon, lung, thyroid, and colorectal and pancreatic adenocarcinoma .
- Isoform 3: Similar to Isoform 1, it is expressed in both hematopoietic and non-hematopoietic tissues .
- Isoform 4: Present in hematopoietic progenitor cells and bone marrow stromal cells .
CD164 is involved in several key biological functions:
- Cell Adhesion: It mediates the adhesion of hematopoietic progenitor cells to the bone marrow stroma, which is essential for their maintenance and differentiation.
- Migration: CD164 plays a role in the migration of these progenitor cells, facilitating their movement within the bone marrow and to other tissues.
- Cancer Progression: The expression of CD164 in various cancers, such as prostate cancer, suggests it may be involved in tumor progression and metastasis .
Recombinant human CD164 is produced using DNA sequences encoding the extracellular domain of the protein. This recombinant form is often tagged with a polyhistidine tag for purification purposes and is expressed in HEK293 cells . The recombinant protein is typically lyophilized from sterile PBS and can be reconstituted for various experimental applications .
Recombinant CD164 is used in research to study its role in cell adhesion, migration, and cancer progression. It is also valuable in investigating the molecular mechanisms underlying hematopoiesis and the maintenance of hematopoietic progenitor cells.
