CD27 Antibody
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Description
Introduction to CD27 Antibody
CD27 is a type I transmembrane protein expressed on naïve and activated T cells, natural killer (NK) cells, and activated B cells . Its ligand, CD70, triggers signaling pathways that enhance T cell proliferation, cytokine production, and long-term immunity . CD27 antibodies are engineered to either agonize or block this pathway, making them promising candidates for immunotherapy in oncology and infectious diseases .
Mechanism of Action
CD27 antibodies function by binding to CD27, mimicking or inhibiting its interaction with CD70. Key mechanisms include:
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Co-stimulation of T cells: Agonist antibodies (e.g., 1F5/CDX-1127) amplify TCR signaling, leading to NF-κB and MAPK8/JNK activation, which drives T cell clonal expansion and survival .
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Fc receptor dependence: Agonist activity requires Fcγ receptor cross-linking, as demonstrated by reduced efficacy in aglycosylated antibody variants .
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Apoptosis regulation: CD27-binding protein SIVA can induce apoptosis, but agonist antibodies primarily override this effect to promote immune activation .
Preclinical Research Findings
Studies in transgenic mice and humanized models highlight the therapeutic potential of CD27 antibodies:
Clinical Development
The lead candidate, CDX-1127 (1F5), is a fully human IgG1 agonist antibody in Phase I trials for advanced cancers . Preclinical data show:
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Synergy with vaccines: Combined use with protein vaccines amplifies CD8+ T cell responses .
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Durable immunity: Treated mice developed protective memory against tumor rechallenge .
Applications in Immunotherapy
CD27 antibodies have broad therapeutic implications:
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Antitumor immunity: Enhanced cytotoxic T cell activity against lymphoma, melanoma, and thymoma .
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Antiviral responses: CD27 costimulation improves CD8+ T cell memory in murine viral models .
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Autoimmune modulation: Potential to suppress pathological T cell activation (under investigation) .
Technical Data and Research Tools
The anti-CD27 antibody ab175403 (Abcam) is widely used in research:
| Parameter | Details |
|---|---|
| Host species | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB (1:3000), IHC (1:100), IF (1:100) |
| Key validation | Bands at ~29 kDa in Raji, Jurkat, and mouse thymus lysates (Western blot) |
| Tissue staining | Localized in thymus and spleen (human, mouse, rat) |
Product Specs
Buffer
PBS with 0.1% Sodium Azide, 50% Glycerol, pH 7.3. Store at -20°C. Avoid repeated freeze-thaw cycles.
Lead Time
Typically, we can ship your orders within 1-3 business days of receiving them. Delivery times may vary depending on your location and the shipping method. For specific delivery times, please consult your local distributors.
Synonyms
CD27; TNFRSF7; CD27 antigen; CD27L receptor; T-cell activation antigen CD27; T14; Tumor necrosis factor receptor superfamily member 7; CD antigen CD27
Target Names
Uniprot No.
Target Background
Function
CD27 is a receptor for CD70/CD27L. It plays a role in the survival of activated T-cells and may contribute to apoptosis through its association with SIVA1.
Gene References Into Functions
- CD27 expression might be a marker for poor prognosis in non-ETV6-RUNX1 B-cell precursor acute lymphoblastic leukemia. PMID: 28649984
- Research indicates that CD95 (Fas) expression is differentially regulated in CD27(+) B-cells from cirrhotic patients. This differential expression is inversely correlated with the frequency of peripheral CD27(+) B-cells and suggests that these memory B-cells are more susceptible to Fas-induced apoptosis in an activation-dependent manner, possibly influenced by endotoxin. This may contribute to the reduced frequency of circulating memory B-cells in cirrhosis. PMID: 27857173
- Preclinical studies suggest that CARs targeting CD70-expressing tumors, which incorporate CD27, may be safe and effective. PMID: 27803044
- The three-dimensional structure of CD27 in complex with a neutralizing, non-competing antibody has been determined. PMID: 28471362
- High CD27 expression has been linked to B-cell lymphoma. PMID: 28108506
- Activated CD70-expressing lymphocytes may trigger CD27 on acute myeloid leukemia (AML) blasts or stem/progenitor cells. PMID: 28031480
- The CD70-CD27 pathway appears to be critical for Epstein-Barr virus (EBV)-specific T cell immunity and broadly for immune surveillance of B cells. This pathway may be a target for immunotherapy of B cell malignancies. PMID: 28011863
- Research has shown elevated levels of soluble CD27 (sCD27) in patients with active vitiligo. PMID: 27556155
- Studies have shown that sCD27 in the cerebrospinal fluid of patients with clinically isolated syndrome (CIS) is associated with a diagnosis of multiple sclerosis and a higher relapse rate. PMID: 28055081
- Findings indicate a potential new role for B cells in dengue pathogenesis, and sCD27 and sCD38 are novel biomarkers linked to clinical outcomes during dengue virus infection. PMID: 27467579
- Novel mutations in TNFRSF7/CD27 have been identified as causes of EBV-associated lymphoproliferative disease/hemophagocytic lymphohistiocytosis, Hodgkin lymphoma, uveitis, and recurrent infections. PMID: 25843314
- NFKB1, CD27, LAG3, and IKZF3 have been identified as new susceptibility genes for psoriasis. PMID: 25006012
- Research provides the first evidence for differential expression of CD27 among chronic lymphocytic leukemia (CLL) prognostic groups, suggesting a role for ZAP-70 dependent signaling in CD27 induction and implicating CD27 in cell-cell interactions with the lymphoid tissue microenvironment. PMID: 26002513
- Multiple layers of memory B cells to T cell-dependent antigens describe immunoglobulin IgM+ B cells as a memory reservoir that can re-enter the germinal center, ensuring replenishment of class-switched memory CD27+ B cells. PMID: 24965774
- Data indicate a unique expansion of CD27(high) plasmablasts in both the cerebrospinal fluid and periphery of transverse myelitis (TM) patients. PMID: 23594958
- CD27-positive B cells from common variable immunodeficiency (CVID) patients are more sensitive than CD27-negative B cells to spontaneous apoptosis and less sensitive to rescue from apoptosis. These B cells fail to differentiate into memory or plasma cells. PMID: 23738704
- A lack of functional CD27 predisposes individuals to a combined immunodeficiency associated with potentially fatal EBV-driven hemophagocytosis, lymphoproliferation, and lymphoma development. PMID: 22801960
- The CCR7+CD45RA-CD27+CD28+ central memory subset is significantly decreased in the peripheral blood CD4+ T cells from rheumatoid arthritis patients. PMID: 23747721
- Research has identified soluble CD27 as an immune modulator associated with enhanced human T cell activation. PMID: 23677477
- These findings emphasize the importance of the CD27-CD70 costimulation pathway for the development of CMV-specific T cell immunity during acute and persistent infection. PMID: 23576505
- The expression of CD27 together with CD44 shows a relationship with several established risk factors as well as response to therapy, indicating the biological significance of these molecules in acute lymphoblastic leukemia. PMID: 23263849
- CD27 and CD28 costimulatory receptors play central roles in the differentiation and activation of gamma-delta T lymphocytes. (Review) PMID: 23456842
- IgM(+)IgD(+)CD27(+) but not switched B cells were significantly reduced in MYD88-, IRAK-4-, and TIRAP-deficient patients, but not UNC-93B-deficient patients. PMID: 23002119
- Serum sCD27 levels are associated with CD27 expression on lymphoma cells and may play a role in diffuse large B-cell lymphoma. PMID: 22280534
- These results suggest that CD27 and CD70 gene polymorphisms may influence the risk of breast cancer and show that some SNPs are associated with breast cancer characteristics in a northern Chinese population. PMID: 22399187
- Decreased CD27 expression on B lymphocytes has been observed in patients with primary hepatocellular carcinoma. PMID: 22429370
- Gene deficiency is associated with combined immunodeficiency and symptomatic EBV viremia. PMID: 22197273
- Down-regulation of CD27 on Mycobacterium tuberculosis (MTB)-specific CD4 T cells could be used as a biomarker for active TB, potentially preceding clinical TB disease. PMID: 22087280
- Data show that in a mouse model of chronic myelogenous leukemia, BCR/ABL+ leukemia stem and progenitor cells express CD27. This reveals a mechanism by which adaptive immunity contributes to leukemia progression. PMID: 22232214
- Data show that CD27(+)/IgD(-)/ZAP70(+) memory B cells accumulate preferentially in the joints of rheumatoid arthritis (RA), suggesting a dynamic maturation of the B cells in this compartment. PMID: 21607290
- CD27 signaling influences T(H) cell differentiation. PMID: 21277898
- CD27(+) lymphocytes represent abnormal mononuclear cell populations in atrophic oral lichen planus (OLP) lesions, suggesting that forms of OLP might exist with different pathogenesis despite similar histology and clinical behavior. PMID: 21237436
- No correlation has been found between CD27 expression on T-lymphocytes and serum levels in children with asthma. PMID: 20800938
- CD27 may play a role in infection in systemic lupus erythematosus (SLE). PMID: 20879061
- CD27 and CD70 constitute a unique ligand and receptor pair that can activate innate and adaptive immunity and regulate immunity versus tolerance. (Review) PMID: 20699361
- These data collectively establish a novel role for the CD70-CD27 axis in human gammadelta T-cell activation, opening new perspectives for its modulation in clinical settings. PMID: 21182090
- Compared with tuberculin-positive controls, patients with bacterial culture-positive pulmonary TB had significantly reduced CD27 expression on antigen-specific CD4 T cells. PMID: 20393787
- A higher proportion of protein-expressing B-cells has been observed in the blood of patients with SLE. PMID: 19866343
- The CD70-CD27 interaction may play a significant role in inducing effective immune responses in dendritic cell-based therapies. PMID: 20201989
- SLE flares may relate to the retention of CD27+ memory B cells. (review) PMID: 15230280
- Functionally differentiated Vgamma9delta2+ T cells lack CD27 expression and are found in reduced numbers in immunocompromised hosts (such as healthy newborns and HIV-infected adults) and during active pulmonary tuberculosis. PMID: 11801693
- Increased levels of soluble CD27 in the cerebrospinal fluid are not diagnostic for leptomeningeal involvement by lymphoid malignancies. The positive predictive value of sCD27 determination for LI was only 54%, but the negative predictive value was 92%. PMID: 11976819
- Intragraft gene expression is a risk factor for acute cardiac allograft rejection. PMID: 12009595
- IL-10 enhances B-cell IgE synthesis by promoting differentiation into plasma cells, a process that is inhibited by CD27/CD70 interaction. PMID: 12197885
- CD27 and CD40 co-stimulatory signals regulated the p53-amplified apoptotic pathway in B cells through the inhibition of the p53-independent apoptotic pathway primarily induced by BCR ligation. PMID: 12324477
- CD27(-) effector CD8(+) T cells might be required for adequate control of chronic viral infection and prevention of disease development. PMID: 12464570
- CD148 and CD27 are expressed in a wide range of B cell non-Hodgkin's lymphomas and do not serve to distinguish between neoplastic cells of naive and memory B cell derivation. PMID: 12685844
- In indolent lymphomas, sCD27 proved to be a powerful marker to predict progression-free survival (p = 0.008). PMID: 12743427
- Signals through CD70 in B lymphocytes of CD70 transgenic mice result in enhanced cell cycle entry while preventing differentiation into IgG-secreting plasma cells. PMID: 15356138
- In primary biliary cirrhosis, CpG stimulation led to a markedly high frequency of intracellular IgM-positive B cells, associated with high levels of synthesized IgM and identified to be a function of CD27(+) memory B cells. PMID: 15685542
Database Links
Involvement In Disease
Lymphoproliferative syndrome 2 (LPFS2)
Subcellular Location
Membrane; Single-pass type I membrane protein.
Tissue Specificity
Found in most T-lymphocytes.
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