CD3E Recombinant Monoclonal Antibody
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Description
Molecular Structure and Target Specificity
The CD3 complex consists of five subunits (γ, δ, ε, ζ, η) with molecular weights ranging from 16–28 kDa. The ε chain (CD3E), encoded by the CD3E gene on chromosome 11q23, is a 207-amino-acid transmembrane protein essential for TCR assembly and signal transduction . Recombinant monoclonal antibodies like clone SK7 (Biotium) and 6G6K9 (Thermo Fisher) bind specifically to the extracellular domain of CD3ε, enabling precise targeting without cross-reactivity to other CD3 subunits .
Mechanisms of Action
CD3E antibodies modulate T-cell activity through two primary mechanisms:
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Diagnostic/Research Use: Bind stably to surface CD3ε without activating T-cells, enabling flow cytometry identification of T-cell populations .
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Therapeutic Use: Engineered Fc-silent variants (e.g., Teplizumab) or bispecific formats (e.g., Catumaxomab) induce controlled T-cell activation or redirected cytotoxicity .
For example, Teplizumab—a humanized anti-CD3E monoclonal antibody—delays type 1 diabetes progression by resetting autoreactive T-cells . Bispecific antibodies like CD3E-CD20 engage T-cells to lyse B-cell malignancies .
Flow Cytometry
Biotium’s SK7 antibody demonstrates validated performance in identifying T-cells across tissues (peripheral blood, spleen, lymph nodes) with multiple fluorescent conjugates :
Therapeutic Development
Recent advances include:
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Immunotoxins: S-CD3e-IT (streptavidin-ZAP conjugate) shows reduced vascular leakage compared to first-gen counterparts .
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T-cell Engagers (TCEs): Bispecific antibodies (e.g., hCD3.1-mCD20) achieve >90% B-cell depletion in preclinical models .
Future Directions
Emerging applications focus on:
Product Specs
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Lead Time
Generally, we can ship the products within 1-3 business days after receiving your orders. Delivery time may vary depending on the purchasing method or location. Please consult your local distributors for specific delivery times.
Synonyms
CD3 epsilon antibody; CD3e antibody; CD3e antigen antibody; CD3e antigen epsilon polypeptide (TiT3 complex) antibody; CD3E antigen epsilon polypeptide antibody; CD3E antigen, epsilon subunit antibody; CD3e molecule epsilon antibody; CD3e molecule, epsilon (CD3 TCR complex) antibody; CD3e molecule, epsilon (CD3-TCR complex) antibody; CD3E_HUMAN antibody; IMD18 antibody; T cell antigen receptor complex epsilon subunit of T3 antibody; T cell surface antigen T3/Leu 4 epsilon chain antibody; T cell surface glycoprotein CD3 epsilon chain antibody; T-cell surface antigen T3/Leu-4 epsilon chain antibody; T-cell surface glycoprotein CD3 epsilon chain antibody; T3E antibody; TCRE antibody
Target Names
Uniprot No.
Target Background
Function
The CD3E molecule is a critical component of the TCR-CD3 complex, found on the surface of T-lymphocytes. This complex plays a pivotal role in the adaptive immune response. When antigen-presenting cells (APCs) activate the T-cell receptor (TCR), the CD3 chains (CD3D, CD3E, CD3G, and CD3Z) transmit the TCR-mediated signals across the cell membrane. All CD3 chains possess immunoreceptor tyrosine-based activation motifs (ITAMs) within their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, leading to the activation of downstream signaling pathways. Beyond its role in signal transduction during T-cell activation, CD3E is essential for proper T-cell development. It initiates the assembly of the TCR-CD3 complex by forming two heterodimers, CD3D/CD3E and CD3G/CD3E. CD3E also participates in the internalization and downregulation of TCR-CD3 complexes from the cell surface through endocytosis sequences located in the CD3E cytosolic region.
Gene References Into Functions
- The ionic interaction between CD3-epsilon and Lck regulates the phosphorylation level of the T-cell receptor. PMID: 28659468
- A novel pathogenic frameshift variant of the CD3E gene has been identified in two unrelated T-B+ NK+ severe combined immunodeficiency infants from Turkey. PMID: 28597365
- The stalk domains of NKp30 and NKp46, which utilize CD3zeta for signaling, were not interchangeable without significant deficiencies in protein folding, plasma membrane targeting, and/or ligand-induced receptor signaling. PMID: 27754869
- The inducible recruitment of WASp to the TCR-CD3 complex is partially dependent on tyrosine phosphorylation of Cd3e. PMID: 26342115
- Our findings indicate that changes in CD3zeta-chain expression from baseline levels are an independent predictor of residual and recurrent head and neck squamous cell carcinoma. PMID: 26888626
- Data suggest that the HIV-1 gp41 transmembrane domain (TMD) directly interacts with the TMDs of the T-cell receptor and its CD3-antigen co-receptors (delta, gamma, and epsilon). These interactions appear to be involved in the immune evasion mechanism of HIV-1. PMID: 26828096
- The recruitment of Nck to the TCR is crucial for mounting an efficient T cell response in vivo. The Nck-CD3epsilon interaction may represent a target for pharmacological modulation of the immune response. PMID: 24470497
- ABCB1 homozygous 3435 TT carrier subjects exhibited the lowest Pgp activity compared to 3435 CT and CC carriers in renal transplant patients. PMID: 23216707
- Local alterations in the lipid composition of TCR microclusters make the CD3epsilon cytoplasmic domain accessible during the early stages of T cell activation. PMID: 23166358
- Results indicate that levels of CD3epsilon, CD25, CD68, and ICAM-1 mRNA in BCC biopsies may predict the risk for new basal cell carcinomas. PMID: 21980389
- Analysis of the transgenic integration site in immunodeficient tgepsilon26 human CD3epsilon transgenic mice. PMID: 21203507
- Findings suggest that the generation of CD3varepsilon chain isoforms with different N-terminal sequences and pI is a common phenomenon. PMID: 19616027
- The recruitment of Nck by CD3 epsilon reveals a ligand-induced conformational change essential for T cell receptor signaling and synapse formation. PMID: 12110186
- The T cell receptor can be recruited to a subset of plasma membrane rafts, independently of cell signaling and concurrently with raft clustering. PMID: 12499387
- Severe Combined Immunodeficiency (SCID) is caused by a CD3E deficiency. PMID: 15546002
- CD3 expression was strong in normal proximal and distal tubular epithelium and in renal oncocytomas, weak in chromophobe carcinoma, and absent in clear cell carcinomas, papillary renal cell carcinoma, and a transitional cell carcinoma. PMID: 16308105
- The CD3 epsilon immune recognition receptor cytoplasmic domain binds to acidic and mixed phospholipid vesicles with a binding strength that correlates with the protein net charge and the presence of clustered basic amino acid residues. PMID: 17176095
- The CD3epsilon-mediated signal transduction pathway is essential for this transformation process. PMID: 17507663
- Notch-dependent cytoplasmic CD3 expression can only be achieved during the early phase of NK-cell differentiation. PMID: 17630354
- In lung adenocarcinoma patients, significant decreases in mean fluorescence intensity (MFI) values for CD3epsilon, but not CD3zeta, were observed in CD4+T and CD8+T cells from pleural effusion compared to peripheral blood, and in peripheral blood of patients compared to healthy donors. PMID: 17668204
- Data show that Nck forms a complex with an atypical PxxDY motif of the CD3epsilon tail, which encompasses Tyr166 within the activation motif and a T-cell receptor endocytosis signal. PMID: 18555270
- Results revealed that the anti-CD3 monoclonal antibody (MAb)-mediated chimpanzee T-cell activation is a function of the anti-CD3 MAb isotype and is not governed by Siglec expression. PMID: 18667496
- Results indicated that the human CD3 epsilon subunit forms a homodimer structure, providing insights into the molecular assembly of the CD3 molecular complex. PMID: 19724882
Database Links
Involvement In Disease
Immunodeficiency 18 (IMD18)
Subcellular Location
Cell membrane; Single-pass type I membrane protein.
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