CD40 Antibody, FITC
Description
Antitumor Mechanisms
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NK Cell Activation: Anti-CD40-FITC (FGK 45.5 clone) increased NK cytolytic activity by 300% against YAC-1 lymphoma cells in BALB/c mice through enhanced IFN-γ production .
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ADCC Enhancement: Fc-engineered XmAbCD40 demonstrated 150-fold greater ADCC potency vs native IgG1 against RPMI8266 myeloma cells (EC50 0.08 ng/mL) .
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Superagonist Design: Fusion of CD40L to anti-CD40 antibodies (e.g., 11B6 clone) reduced effective dose by 90% while increasing IL-12p70 secretion 8-fold in human dendritic cells .
B-Cell Regulation
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Isotype Switching: HM40-3 clone induced IgM-to-IgG switching at 10 ng/mL when combined with IL-4 in murine splenocyte cultures .
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Apoptosis Resistance: Pre-treatment with 1C10 clone reduced B-cell apoptosis by 65% in CD40L-deficient models .
Flow Cytometry Protocols
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Multipanel Compatibility: Compatible with PE-Cy7 (575 nm), APC (660 nm), and BV421 (420 nm) channels without spectral overlap .
Functional Studies
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T-cell Priming: HI40a-FITC identified CD40+ dendritic cells responsible for 83% of antigen-specific T-cell proliferation in melanoma models .
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Ligand Blocking: G28.5 clone inhibited CD40-CD154 interaction by 94% at 10 µg/mL in co-culture assays .
Technical Considerations
| Parameter | Specification | Impact on Results |
|---|---|---|
| Storage | 2-8°C in dark; avoid freeze-thaw | >90% fluorescein retention at 6mo |
| Titration Range | 0.1-5 µg/mL | EC50 varies by clone (±0.2 log) |
| Cross-Reactivity | <5% with TNFRSF1A, FAS, OX40 | Validated via knockout cell lines |
Product Specs
Product Science Overview
CD40 is a 48 kD type I transmembrane glycoprotein, also known as Bp50, and is a member of the tumor necrosis factor receptor (TNFR) superfamily . It is expressed on various cell types including B cells, basal epithelial cells, macrophages, follicular dendritic cells, endothelial cells, and a subset of CD34+ hematopoietic progenitors . CD40 plays a crucial role in the regulation of B cell development and maturation, immunoglobulin (Ig) isotype switching, and in protecting B cells from apoptosis induced by surface Ig .
FITC (Fluorescein Isothiocyanate) is a fluorescent dye commonly used in flow cytometry and other fluorescence-based techniques. When conjugated to antibodies, FITC allows for the visualization and quantification of specific cell surface markers . The FITC Mouse Anti-Human CD40 antibody is a monoclonal antibody that has been conjugated with FITC to enable the detection of CD40 expression on human cells .
The FITC Mouse Anti-Human CD40 antibody is widely used in research to study the roles of CD40 in immune responses, particularly in B cell biology . It is commonly used in flow cytometry to analyze the expression of CD40 on various cell types, including peripheral blood lymphocytes . This antibody is also useful for investigating the mechanisms of B cell activation, proliferation, and differentiation .
