CD5 Monoclonal Antibody
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Description
Biological Role of CD5
CD5 modulates T-cell receptor (TCR) and B-cell receptor (BCR) signaling, acting as a negative regulator of immune activation. Key features include:
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Expression: Found on 95% of thymocytes, 72% of peripheral T cells, and B1a cells .
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Ligand Interaction: Binds CD72 on B cells, influencing T-B cell crosstalk and proliferation .
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Pathological Relevance: Overexpressed in chronic lymphocytic leukemia (CLL), mantle cell lymphoma, and T-cell malignancies .
Key CD5 Monoclonal Antibody Clones
Diagnostic Applications
CD5 monoclonal antibodies are critical in distinguishing hematologic malignancies:
Staining Patterns:
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Normal Tissues: Membranous staining in T-cell zones (tonsils, lymph nodes) and B1a cells .
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Tumors: Strong membranous expression in CLL and partial positivity in colorectal cancer .
Immune Checkpoint Blockade
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Anti-CD5 in Cancer: Blocking CD5 enhances cytotoxic T-cell activity against tumors. In murine models, anti-CD5 monoclonal antibodies delayed 4T1 breast tumor growth by increasing CD8+/CD4+ T-cell ratios and activation markers (CD69, Fas) .
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CAR T/NK Cell Therapy: CD5-targeted CAR NK-92 cells eliminated CD5+ T-cell malignancies in xenograft models .
Autoimmunity
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Preclinical studies suggest CD5 blockade reduces self-reactive T-cell activity, potentially treating autoimmune disorders .
Research Findings
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Mechanistic Insights:
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Gene Expression: RNA-seq data confirm CD5 expression in lymphoid tissues and epithelial subsets (e.g., bronchial glands) .
Validation and Specificity
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Orthogonal Strategies: Antibodies like MSVA-005R align with RNA expression data from the Human Protein Atlas .
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Cross-Reactivity: Clone UCHT2 recognizes primate CD5 but not murine homologs, while 53-7.3 is mouse-specific .
Challenges and Future Directions
Product Specs
Buffer
Phosphate-buffered saline (PBS), pH 7.4, containing 0.02% sodium azide as a preservative and 50% glycerol.
Form
Liquid
Lead Time
Typically, we can ship products within 1-3 business days of receiving your order. Delivery times may vary depending on the purchase method and location. Please consult your local distributor for specific delivery details.
Synonyms
CD5; LEU1; T-cell surface glycoprotein CD5; Lymphocyte antigen T1/Leu-1; CD antigen CD5
Target Names
Uniprot No.
Target Background
Function
CD5 may act as a receptor in regulating T-cell proliferation.
Gene References Into Functions
- Current findings support the notion that CD5 is not just a phenotypic marker but plays a significant role in Chronic Lymphocytic Leukemia (CLL) cell biology, as demonstrated by the impact of germline-defined functional CD5 variants on CLL outcome. PMID: 26991857
- ZAP70 is the only gene that is differentially expressed in CD5-high and CD5-low normal B-lymphocytes, highlighting the crucial role of Zap-70 tyrosine kinase in BCR signaling alterations in B-CLL. PMID: 29125235
- Elevated CD5 plasma levels in patients with severe Autism Spectrum Disorder (ASD) suggest potential involvement of CD5 in autism physiology. However, further research with larger populations is necessary to determine whether this increase is merely a consequence of autism or plays a pathogenic role in the disease. PMID: 28406773
- A CD34-negative MYC-rearranged B-lymphoblastic lymphoma aberrantly expresses CD3 and CD5. PMID: 28280048
- This study provides insights into the regulation of CD5 expression on leukemic T cells, which may aid in understanding the molecular mechanism of CD5 down-regulation. PMID: 28752543
- CD5 transcription is increased, resulting in the production of three mRNA isoforms through alternative polyadenylation (APA), all contributing to protein production at varying levels. PTBP1 binds in the vicinity of pA1, leading to an increase in mRNA levels. Additionally, miR-204 targets the longer CD5 mRNA. PMID: 27005442
- High CD5 expression is associated with lymphoma in Waldeyer's ring. PMID: 27616053
- Trans-signaling of IL-6 can only occur via soluble IL-6 receptor (sIL-6R), but not soluble CD5 (sCD5). PMID: 28159554
- A significant proportion of CD5(low) B cells in patients are leukemic, identifying CD5(low) B cells as a crucial component of CLL with implications for CLL pathogenesis, clinical monitoring, and the development of anti-CD5-directed therapies. PMID: 27693386
- The CD5 Pro224-Ala471 haplotype in homozygous state improved melanoma survival in the entire patient cohort (HR = 0.27, 95% CI 0.11-0.67, Adj. p = 0.005). These findings highlight the importance of genetic variability in immune-related genes for clinical outcomes in melanoma. PMID: 27169428
- R-EPOCH therapy does not seem to affect the known poorer prognosis of patients with de novo CD5+ diffuse large B-cell lymphoma, and CD5 expression remained an independent prognostic factor in R-EPOCH-treated patients with diffuse large B-cell lymphoma. PMID: 28039906
- CD5+B lymphocytes were increased and activated in autoimmune hemolytic anemia patients. PMID: 26968550
- This study confirms the poor prognosis of de novo CD5+ DLBCL in a large multicenter cohort despite initial rituximab-containing chemotherapy and suggests that stem cell transplantation fails to salvage the majority of these patients. PMID: 26800311
- 5.2% were positive for CD5 and 5.7% positive for Epstein-Barr virus encoded RNA. PMID: 26369546
- CD5 polymorphisms are associated with systemic lupus erythematosus. PMID: 25402503
- Three tyrosine-based domains within the CD5 cytoplasmic tail promote T-cell survival through non-overlapping mechanisms. PMID: 26363459
- A case of mantle cell lymphoma with a unique pattern of CD5 negative to positive expression switching in relapse. PMID: 24166616
- Useful in distinguishing thymic carcinoma from poorly differentiated lung carcinoma. PMID: 23958552
- Data shows that the proportion of CD5+ B cells at 1 month of age is inversely correlated to total IgG levels at 18 and 36 months of age. PMID: 24928995
- Down-regulation of CD5 on activated CD8(+) T cells may serve as a useful marker of dysregulated T cell activation and proliferation in familial hemophagocytic lymphohistiocytosis type 2 (FHL2). PMID: 24051121
- CD5 expression in nodal marginal zone lymphoma correlates with a higher frequency of dissemination, but patients have an indolent clinical course and excellent overall survival. PMID: 24124149
- Our results indicate that CD5(+) B cell subsets might be associated with the pathogenesis of secondary progressive multiple sclerosis (SPMS). PMID: 22732449
- Functional CD5-dependent casein kinase 2 signaling is necessary in a CD5 knock-in mouse for efficient differentiation of naive CD4+ T cells into T helper (Th)2 and Th17 cells, but not Th1-type cells. PMID: 22904299
- Case Report: CD5-positive marginal zone B-cell lymphoma of the mucosa-associated lymphoid tissue (MALT) of the lung. PMID: 22333190
- Our findings show that CD5 expression is infrequent in MALT lymphoma and is often associated with nongastric disease and an increased tendency to present with disseminated disease. PMID: 22406370
- Hepatitis C virus infection of human T lymphocytes is mediated by CD5. PMID: 22278227
- The primary carcinoma showing thymus-like elements of the thyroid frequently expresses KIT and CD5 proteins. PMID: 21835435
- Alternative costimulation via CD5, rather than classic costimulation via CD28, primes naive T cells for stable Th17 development by promoting the expression of IL-23R. PMID: 21926348
- Post-thymic regulation of CD5 levels in human memory T cells is inversely associated with the strength of responsiveness to interleukin-15. PMID: 21539877
- Immunological significance of CD19 for the IL-10 production by CD5(+) B cells. PMID: 21786452
- Case Report: Describing the recurrence of CD5-positive MALT lymphoma in the oral cavity and comparing the immunohistochemical expressions of CD5, lambda, and kappa between the primary and recurrent tumors. PMID: 21892966
- CD5 glycoprotein-mediated T cell inhibition dependent on inhibitory phosphorylation of Fyn kinase. PMID: 21757751
- Conclude that high percentages of CD5+B cells independently associate with an increased risk of early conversion to multiple sclerosis in clinically isolated syndrome patients. PMID: 21436320
- Data shows that CD5 expression is associated with NFAT2 activity and mild STAT3 activity, indicating that CD5 controls IL-10 secretion. PMID: 21398617
- The full-length protein variant, encoded by E1A-cd5, translocates the phosphatase SHP-1 to the vicinity of the B-cell receptor, raises its threshold, and thereby limits the response of autoreactive B cells [review]. PMID: 21395509
- CD5-positive splenic marginal zone lymphoma may be related to memory B-cell neoplasm or plasma cell differentiation. PMID: 21123968
- Type B3 thymic epithelial tumor in an adolescent detected by immunohistochemical staining for CD5, CD99, and KIT (CD117): a case report. PMID: 19901887
- CD5-negative regulation of B cell receptor signaling originates from tyrosine residue Y429 outside an immunoreceptor tyrosine-based inhibitory motif. PMID: 11751967
- Origins and functions of B-1 cells with notes on the role of CD5. Review. PMID: 11861604
- Multiple somatic mutations in human cancers. The value of the CD5 microsatellite as a marker for instability in different tumor types, particularly in B-cell leukemia and lymphoma. PMID: 11869933
- CD5 supports the survival of B cells by stimulating IL-10 production and by concurrently exerting negative feedback on BCR-induced signaling events that can promote cell death. PMID: 12393419
- CD5-negative, CD10-negative small B-cell leukemia: variant of chronic lymphocytic leukemia or a distinct entity. PMID: 12447961
- CD5 is rapidly recruited at the immunological synapse (IS) and lowers the T cell response elicited by antigen presentation by targeting downstream signaling events without affecting IS formation. PMID: 12707340
- The CD5+ diffuse large-B-cell lymphoma (DLBCL) constitutes a disease category distinct from that of CD5- DLBCL and other CD5+ malignancies. PMID: 14603444
- The 5'-flanking region of human CD5 is transcriptionally active in T cells, and Ets transcription factors in conjunction with other regulatory elements are responsible for constitutive and tissue-specific CD5 expression. PMID: 15187131
- Overexpression in these cells indicates disease progression in B-cell leukemia patients. PMID: 15549146
- CD5 is associated with receptor revision in activated mature B cells and likely to promote expression of suitable immunoglobulin gene receptors capable of initiating the germinal center reaction. PMID: 15843554
- The polymorphic CD5 promoter is associated with increased susceptibility to B-cell chronic lymphocytic leukemia and mantle cell lymphoma. PMID: 15981803
- Balance between the 2 alternative exons 1 might be central to the regulation of membrane CD5 in human B cells. PMID: 15998834
- Analysis of CD5-positive diffuse large B-cell lymphoma with the unusual phenotype of cytoplasmic CD20 (+), surface CD20 (-). [case report] PMID: 16923582
Database Links
Subcellular Location
Cell membrane; Single-pass type I membrane protein.
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