CD7 Human, Sf9
Description
Molecular Structure and Design
CD7 Human, Sf9 comprises a 155-amino-acid extracellular domain (residues 26–180) fused with a C-terminal hexahistidine (6xHis) tag for purification . Key structural features include:
The protein's immunoglobulin-like domain mediates interactions with ligands such as SECTM1 and galectin-1 .
Production and Purification
CD7 Human, Sf9 is synthesized using Sf9 cells adapted for serum-free suspension culture in media such as ESF 921 or ESF AF . The workflow involves:
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Baculovirus Transduction: Sf9 cells are infected with recombinant baculovirus carrying the CD7 gene .
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Harvesting: Secreted protein is collected from culture supernatant.
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Chromatography: Nickel-affinity chromatography isolates the His-tagged protein .
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Formulation: Stabilized in PBS (pH 7.4) with 10% glycerol, requiring storage at -20°C .
T-Cell Activation Studies
CD7 Human, Sf9 binds to SECTM1 and co-stimulates T-cell proliferation and integrin-mediated adhesion . It enhances:
Therapeutic Development
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Immunotoxins: Anti-CD7 scFv-PE24 immunotoxins produced in CHO cells show potent cytotoxicity against CD7+ leukemia cells (IC<sub>50</sub>: 15–156 pM) .
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Apoptosis Induction: Galectin-1 binding to CD7 triggers caspase-dependent T-cell death, relevant for treating inflammatory diseases .
Disease Modeling
CD7 is overexpressed in T-cell acute lymphoblastic leukemia (T-ALL) and acute myeloid leukemia (AML), making CD7 Human, Sf9 a target for:
Comparative Analysis of CD7 Variants
Research Limitations
Product Specs
T-cell antigen CD7, GP40, LEU-9, Tp40, TP41, CD7, T-cell leukemia antigen, T-cell surface antigen Leu-9.
Sf9, Baculovirus cells.
ADLAQEVQQS PHCTTVPVGA SVNITCSTSG GLRGIYLRQL GPQPQDIIYY EDGVVPTTDR RFRGRIDFSG SQDNLTITMH RLQLSDTGTY TCQAITEVNV YGSGTLVLVT EEQSQGWHRC SDAPPRASAL PAPPTGSALP DPQTASALPD PPAASALPHH HHHH.
Product Science Overview
CD7, also known as T-cell antigen CD7, GP40, LEU-9, Tp40, and TP41, is a type I transmembrane glycoprotein. It is primarily expressed on pluripotential hematopoietic cells, nearly all human thymocytes, and some peripheral blood T cells. The CD7 molecule is notably absent in a subset of human T cells under certain physiological conditions. An increase in CD7-negative T cells can be observed in various inflammatory skin diseases .
The CD7 protein is a single, glycosylated polypeptide chain consisting of 164 amino acids (26-180a.a.) and has a molecular mass of approximately 17.5 kDa. When analyzed using SDS-PAGE, the molecular size appears to be between 18-28 kDa . The recombinant CD7 protein produced in Sf9 Baculovirus cells includes a 6-amino acid His tag at the C-terminus, which facilitates its purification through proprietary chromatographic techniques .
The recombinant CD7 protein is produced in Sf9 Baculovirus cells. Sf9 cells are derived from the fall armyworm (Spodoptera frugiperda) and are commonly used in the baculovirus expression system for producing recombinant proteins. The CD7 protein is expressed as a glycosylated polypeptide chain and is purified to a purity greater than 95.0% as determined by SDS-PAGE .
For short-term storage (2-4 weeks), the CD7 protein should be kept at 4°C. For long-term storage, it is recommended to store the protein at -20°C. To ensure stability during long-term storage, adding a carrier protein such as 0.1% human serum albumin (HSA) or bovine serum albumin (BSA) is advisable. It is important to avoid multiple freeze-thaw cycles to maintain the protein’s integrity .
The recombinant CD7 protein is used in various laboratory research applications. It is important to note that ProSpec’s products, including the CD7 protein, are intended for laboratory research use only and are not suitable for use as drugs, agricultural or pesticidal products, food additives, or household chemicals .
