CDC40 Antibody

Gene and Protein Overview

• Gene: CDC40 is located on human chromosome 6 and encodes a 65,521-Da protein with seven WD repeats, enabling protein-protein interactions .

• Function: CDC40 is essential for catalytic step II of pre-mRNA splicing, a process facilitated by its inclusion in the spliceosome complex. Homology to yeast Prp17 suggests dual roles in splicing and cell cycle progression .

Biological Functions

• Splicing: CDC40 interacts with spliceosome components like CDC5, PRP19, and CWC15, particularly within the NineTeen Complex (NTC) .

• Cell Cycle: Yeast studies link CDC40 homologs to cell division regulation, though mammalian roles remain under investigation .

Role in Disease and Cancer

Lung cancer studies reveal CDC40’s essentiality:

• Knockdown Effects: shRNA-mediated CDC40 depletion in lung cancer cells induces growth arrest, apoptosis, and splicing defects (e.g., intron retention) .

• Therapeutic Potential: CDC40 inhibition shows promise for cancer treatment, though normal cell toxicity (e.g., in MCF10A lines) requires further evaluation .

Research Applications

• Immunoprecipitation: CDC40 antibodies enable co-IP of spliceosome components (e.g., CDC5, PRP19) for interaction mapping .

• Imaging: ICC staining highlights nuclear localization in HeLa cells (Figure 2 ).