CDC6B Antibody
Description
Introduction to CDC6B Antibody
The CDC6B antibody targets the Cell Division Cycle 6 (CDC6) protein, a crucial regulator in the initiation of DNA replication . CDC6 is highly conserved across species and plays a vital role in ensuring accurate genome duplication during cell division . The CDC6 protein is essential for the assembly of pre-replicative complexes (pre-RCs) at the origin of replication, a necessary step for DNA replication to begin .
Characteristics of CDC6B Antibody
Applications in Research
CDC6B antibodies are utilized in various research applications, primarily focusing on understanding DNA replication, cell cycle regulation, and cancer biology . Specific applications include:
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Western Blotting: Used to detect and quantify CDC6 protein levels in cell lysates .
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Immunohistochemistry: Used to visualize CDC6 expression and localization in tissue samples .
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Immunofluorescence: Used to examine the subcellular distribution of CDC6 within cells .
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ELISA: Used to measure the interaction of the antibody with CDC6 .
Role of CDC6 in DNA Replication
CDC6, a protein with high similarity to Saccharomyces cerevisiae Cdc6, is essential for initiating DNA replication . It functions as a regulator in the early stages of DNA replication, localizing in the cell nucleus during the G1 phase of the cell cycle and translocating to the cytoplasm at the start of the S phase . This translocation is regulated by phosphorylation via Cdks . CDC6's transcription is regulated in response to mitogenic signals through transcriptional control mechanisms involving E2F proteins and is crucial for assembling pre-replicative complexes at replication origins .
Antibody Validation
Boster Bio validates CDC6B antibodies using Western blot (WB) applications . Validation processes include testing on known positive control samples to ensure specificity and high affinity .
Significance in Cancer Research
CDC6 is overexpressed in various cancers, making it a potential target for cancer therapy . Research has shown that targeting CDC6 can inhibit DNA replication and induce apoptosis in cancer cells .
