CDC73 Antibody
Description
Introduction to CDC73 Antibody
The CDC73 antibody is a laboratory tool designed to detect and study the CDC73 protein (parafibromin), a tumor suppressor encoded by the CDC73 gene. This protein plays critical roles in transcriptional regulation as part of the PAF1 complex, which associates with RNA polymerase II and histone-modifying enzymes . CDC73 mutations are linked to hyperparathyroidism-jaw tumor syndrome (HPT-JT) and parathyroid carcinomas, making its detection vital for both research and clinical diagnostics .
Key Applications of CDC73 Antibodies
CDC73 antibodies are widely used in molecular and cellular biology for:
-
Western blotting (WB): Detecting endogenous CDC73 protein (~60–61 kDa) .
-
Immunoprecipitation (IP): Isolating CDC73 and its interacting partners (e.g., CPSF, CstF complexes) .
-
Immunohistochemistry (IHC): Assessing CDC73 expression in tumor tissues .
-
Immunofluorescence (IF): Localizing CDC73 within the nucleus or cytoskeleton .
Mechanistic Insights
-
CDC73 interacts with CPSF and CstF complexes, facilitating mRNA 3′ processing and transcriptional elongation .
-
Depletion of CDC73 reduces recruitment of CPSF/CstF subunits to gene loci (e.g., INTS6), impairing mRNA maturation .
-
CDC73 loss correlates with tumor aggressiveness in breast, gastric, and colorectal cancers .
Clinical Associations
Technical Considerations for CDC73 Antibody Usage
-
Storage: Stable at -20°C in PBS with 0.02% sodium azide and 50% glycerol .
-
Validation: Verified via siRNA knockdown, immunoprecipitation, and mass spectrometry .
-
Cross-reactivity: Confirmed in human, mouse, rat, and monkey tissues .
Future Directions and Therapeutic Potential
Current research focuses on:
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- PHP, an accessory subunit of Paf1C, facilitates proper regulation of genes within chromatin enriched with H3K27me3. PMID: 21720211
- CDC73 is crucial for high levels of FLC expression in specific autonomous-pathway-mutant backgrounds. It functions to promote activating histone modifications (H3K4me3) while simultaneously preventing repressive modifications (e.g., H3K27me3). [CDC73] PMID: 20463090
- PLANT HOMOLOGOUS TO PARAFIBROMIN is the plant homolog of parafibromin and interacts physically with other Paf1C components. PMID: 20363855
