CDH8 Antibody

CDH8 Antibody Validation and Specificity

Two widely used CDH8 antibodies have been rigorously validated:

Key validation findings:

• Both antibodies recognize a single band at 135 kDa, matching CDH8’s predicted molecular weight .

• Specificity confirmed via loss-of-function tests in untransfected L-cells (no endogenous cadherin expression) .

• Cross-reactivity: 97% sequence identity with mouse CDH8 and 98% with rat .

Neurological Studies

• Prefrontal Cortex (PFC) and Striatum: CDH8 localizes to synaptic junctions in these regions, suggesting roles in cognitive and motor function .

• Axon Guidance: CDH8 mediates neurite outgrowth in vitro, with antibody-blocking experiments implicating its extracellular domain in adhesion-dependent signaling .

Disease Relevance

While not directly linked to human disorders in the provided sources, CDH8’s synaptic role positions it as a candidate for neurodevelopmental or psychiatric conditions.

Limitations and Considerations

• Cross-Reactivity: No evidence of off-target binding in validated studies, but epitope accessibility may vary by tissue .

• Functional Blocking: CAD8-1 monoclonal antibody disrupts CDH8-mediated adhesion in transfected cells, enabling mechanistic studies .