CDK1/CDK2/CDK3 Antibody
Description
Antibody Properties
The antibody targets a conserved 13-amino-acid sequence surrounding Y15 phosphorylation, which is identical across CDK1, CDK2, CDK3, and CDK5 . Key characteristics include:
Cross-Reactivity and Limitations
A critical limitation is the antibody’s inability to distinguish between CDK1, CDK2, CDK3, and CDK5 due to sequence homology in the Y15 region. This issue is widespread among commercial antibodies :
Research Applications
The antibody is used to study CDK activity in:
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Cell Cycle Regulation: CDK1/CDK2/CDK3 complexes drive G1-S, S-G2, and G2-M transitions. Y15 phosphorylation inhibits CDK activity until dephosphorylated by CDC25 phosphatases .
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DNA Repair: CDK2 phosphorylates BRCA1 and BRCA2, linking cell cycle arrest to DNA repair mechanisms .
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Cancer Biology: Elevated CDK1 Y15 phosphorylation correlates with drug resistance in leukemia and breast cancer by inactivating BAD and promoting mitotic slippage .
