CDK5RAP1 Antibody

Overview of CDK5RAP1 Antibody

CDK5RAP1 antibodies are polyclonal reagents primarily raised in rabbits, designed to target the human CDK5RAP1 protein (UniProt ID: Q96SZ6). This protein functions as a methylthiotransferase, catalyzing post-transcriptional modifications of mitochondrial tRNAs essential for efficient mitochondrial translation . Additionally, CDK5RAP1 interacts with CDK5R1 to regulate CDK5 activation, impacting cellular processes such as apoptosis and proliferation .

Synonyms: HSPC167, C20orf34, CGI-05, mitochondrial tRNA methylthiotransferase .

Research Findings on CDK5RAP1 in Cancer Biology

CDK5RAP1 deficiency has been linked to apoptosis in malignant melanoma (A375 cells) via reactive oxygen species (ROS) and nuclear factor-κB (NF-κB) signaling .

Key Experimental Results:

Mechanistic Insights:

• CDK5RAP1 knockdown increases ROS, activating NF-κB and Bad phosphorylation, which drives mitochondrial apoptosis .

• NF-κB inhibition reverses apoptosis but not ROS elevation, indicating ROS acts upstream .

Applications in Biomedical Research

CDK5RAP1 antibodies are critical for:

• Western Blot: Detecting endogenous CDK5RAP1 in human heart tissue, mouse/rat brain lysates .

• Immunohistochemistry: Localizing protein expression in formalin-fixed paraffin-embedded (FFPE) tissues (e.g., human breast carcinoma) .

• Functional Studies: Investigating mitochondrial translation defects or CDK5-related signaling in cancer .

Protocol Considerations:

• Antigen retrieval with TE buffer (pH 9.0) or citrate buffer (pH 6.0) improves IHC results .

• Titration is recommended for optimal signal-to-noise ratios .