CDKA-1 Antibody
Description
Overview of CDKA-1 Antibody
The CDKA-1 antibody (e.g., ab108290) is a recombinant monoclonal antibody targeting CDK2-associated protein 1 (CDK2AP1), also known as Deleted in Oral Cancer 1 (DOC1). This protein functions as an inhibitor of cyclin-dependent kinase 2 (CDK2) and is a component of the histone deacetylase NuRD complex, which regulates chromatin remodeling . CDKA-1 antibodies are primarily used in research to study cell cycle regulation, cancer biology, and epigenetic modifications.
Primary Applications
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Western Blot (WB): Detects endogenous CDK2AP1 in human cell lysates (e.g., HeLa, HUVEC, LNCaP) .
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Mechanistic Studies: Investigates CDK2AP1’s role in tumor suppression, cell proliferation, and chromatin dynamics .
Key Research Insights
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Functional Role: CDK2AP1 binds to CDK2, inhibiting its kinase activity and modulating cell cycle progression .
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Cancer Relevance: Reduced CDK2AP1 expression correlates with oral and other cancers, suggesting its role as a tumor suppressor .
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Structural Data: The antibody recognizes a 12 kDa protein band in human cell lines, consistent with CDK2AP1’s molecular weight .
Validation Data
| Cell Line | Observed Band Size | Reactivity Confirmed |
|---|---|---|
| 293T | 12 kDa | Yes |
| HeLa | 12 kDa | Yes |
| HUVEC | 12 kDa | Yes |
| LNCaP | 12 kDa | Yes |
Source: ab108290 product validation .
CDK2AP1 in Cell Cycle Regulation
CDK2AP1 regulates the G1/S transition by binding CDK2, preventing its interaction with cyclins and inhibiting phosphorylation of downstream targets like retinoblastoma (Rb) protein . This mechanism is critical for maintaining genomic stability and preventing uncontrolled proliferation.
Chromatin Remodeling
As part of the NuRD complex, CDK2AP1 facilitates histone deacetylation, leading to chromatin condensation and transcriptional repression. Dysregulation of this process is implicated in cancer and developmental disorders .
Limitations and Future Directions
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- CDKA;1 and CYCD3;2 are essential for the terminal division in the stomatal lineage. PMID: 24687979
- Research findings demonstrate a direct link between cell proliferation and the control of the redox state, highlighting the crucial role of CDKA;1 in regulating cellular oxidative stress. PMID: 22098373
- CDKA;1 plays a central role in regulating the plant Retinoblastoma homolog RBR1, a key tumor suppressor protein. Studies indicate that codepletion of RBR1 and CDKA;1 rescues most defects observed in cdka;1 mutants, suggesting a complex interplay between these two proteins in cell cycle regulation. PMID: 22595674
- Expression of a dominant negative CDKA;1 allele under the control of the STM promoter disrupts post-embryonic development. Specifically, inhibiting CDK activity at the shoot apex leads to premature differentiation of shoot apical meristem cells, emphasizing the crucial role of CDKA;1 in maintaining meristematic activity. PMID: 20659279
- When a single cdka;1 sperm is delivered, either female gamete can be fertilized, resulting in similar proportions of seeds containing either a single endosperm or a single embryo. This finding reveals the unique role of CDKA;1 in fertilization and the development of seeds. PMID: 20610483
- Studies have shown that the DNA damage checkpoint in Arabidopsis can operate independently of the phosphorylation of CDKA;1, suggesting alternative mechanisms for DNA damage response in this plant. PMID: 19948791
- CDC2A, a homolog of CDKA;1, plays a critical role in the fertilization process of the endosperm, a nutrient-rich tissue supporting embryonic development. PMID: 16311592
- The balance between cell division and differentiation is meticulously regulated through the intricate interaction between CDKA;1 and the antiphosphatase PAS2. This dynamic interplay is essential for maintaining cellular homeostasis and proper development. PMID: 16698944
- The Arabidopsis thaliana Cdc2(+)/Cdc28 homolog CDKA;1 undergoes phosphorylation in the T-loop, and phosphorylation at the conserved Thr-161 residue is essential for its proper function. PMID: 17369369
- Phosphorylation of T161 in Arabidopsis CDKA;1 is indispensable for cell division during male gametogenesis, highlighting the critical role of this specific phosphorylation event in reproductive processes. PMID: 17764501
