CDSN Antibody
Description
CDSN Antibody Overview
The CDSN antibody targets the corneodesmosin protein (CDSN), a 52-65 kDa glycoprotein essential for epidermal barrier integrity. It is available in polyclonal forms from multiple suppliers, including Proteintech and Assay Genie, with applications in Western blot (WB), immunohistochemistry (IHC), immunofluorescence (IF), and ELISA.
Key Features of Available Antibodies
| Antibody Clone | Immunogen | Reactivity | Applications | Host/Isotype |
|---|---|---|---|---|
| 13184-1-AP (Proteintech) | CDSN fusion protein Ag3854 | Human, Mouse | WB, IHC, IF, ELISA | Rabbit/IgG |
| 27953-1-AP (Proteintech) | CDSN fusion protein Ag27688 | Human, Mouse | WB, IHC, IF, ELISA | Rabbit/IgG |
| CAB14602 (Assay Genie) | CD300A (note: mislabeled as CDSN) | Human | WB | Rabbit/IgG |
Note: The CAB14602 antibody (Assay Genie) appears to target CD300A, not CDSN, suggesting a potential labeling error .
2.1. Cancer Research
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Ovarian Cancer (OC): CDSN is overexpressed in OC tissues and correlates with poor prognosis. Knockdown experiments using the 13184-1-AP antibody demonstrated reduced OC cell proliferation and increased apoptosis, suggesting CDSN as a therapeutic target .
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Immune Microenvironment: High CDSN expression in OC is linked to immune infiltration, particularly in antigen-presenting cells (APCs), as validated by IHC and flow cytometry .
2.2. Skin Disorders
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Psoriasis: Increased CDSN expression is observed in lesional psoriasis, extending into the stratum spinosum compared to normal skin .
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Atopic Dermatitis (AD): Staphylococcus aureus binds to CDSN’s N-terminal region, with higher accessibility in low-NMF corneocytes. Blocking this region with antibodies reduces bacterial adhesion .
2.3. Genetic Insights
The CDSN gene (NCBI Gene ID: 1041) maps to chromosome 6 (NC_000006.12) and encodes a protein critical for corneodesmosome stability. Mutations in CDSN are associated with genodermatoses, including inflammatory peeling skin syndrome .
Recommended Dilutions
| Application | Dilution Range |
|---|---|
| Western Blot (WB) | 1:500–1:3000 |
| Immunohistochemistry (IHC) | 1:150–1:600 |
Antigen Retrieval
4.1. CDSN in Disease Pathogenesis
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Ovarian Cancer: CDSN knockdown reduces cell proliferation by 30–40% in SKOV3 and HEY cells .
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Psoriasis: Lesional skin shows 3-fold higher CDSN immunoreactivity in the stratum spinosum compared to normal skin .
4.2. Diagnostic Potential
CDSN expression levels, detected via IHC, correlate with immune infiltration in OC (AUC > 0.8 for diagnostic accuracy) .
Product Specs
Target Background
Corneodesmosin (CDSN) is a protein that plays a critical role in the structure and function of the epidermis. It is a component of corneodesmosomes, which are specialized cell junctions that hold together the cells of the stratum corneum, the outermost layer of skin. CDSN is essential for the integrity of the epidermal barrier, which protects the body from environmental insults and prevents water loss.
Mutations in the CDSN gene are associated with several skin disorders, including:
- Hypotrichosis simplex of the scalp
- Generalized peeling skin disease
- Netherton syndrome
- Psoriasis
- Ankylosing spondylitis
Research has also shown that CDSN is involved in:
- Hair follicle integrity
- Skin identification
- Amyloidosis
Here are some relevant research studies:
- This research reveals, for the first time, the male-specific association of the PSORS1C3 gene with psoriasis risk and the PSORS1C1/CDSN gene with the severity of the disease. Nevertheless, the age-dependent associations need further validation using larger sample sizes and across diverse populations. PMID: 29589160
- Our findings suggest that a burden of low-frequency coding variants in N4BP2, CDSN, PRTG, and AHRR is linked to an increased risk of nonsyndromic cleft lip with or without cleft palate (NSCL/P). Conversely, low-frequency variants in other genes are associated with a decreased risk of NSCL/P. These results highlight the contribution of low-frequency variants to the genetic etiology of NSCL/P. PMID: 28425186
- This study investigated potential direct protein-protein interactions between six late cornified envelope (LCE) proteins and two corneodesmosin sequence variants. Partial colocalization of LCE2 and CDSN was observed in both normal and psoriasis skin. PMID: 25078048
- We report a case of a patient with peeling skin disease (PSD) caused by a novel homozygous large deletion within the 6p21.3 region, encompassing the CDSN gene. This deletion leads to the complete absence of CDSN expression. PMID: 24116970
- Case Report: a homozygous deletion of CDSN is considered to be responsible for generalized peeling skin disease. PMID: 24794518
- The PSORS1C1/CDSN gene may play a role in the pathogenesis of ankylosing spondylitis. PMID: 24210685
- A nonsense mutation (C717G) in the cDNA sequence of the CDSN gene was identified in all three patients of the family. PMID: 22875505
- The CDSN -619C/T polymorphism may not be associated with susceptibility to psoriasis. PMID: 22033905
- CDSN plays a vital role in maintaining the structural and functional integrity of the epidermis and hair follicle integrity by preventing the rupture of corneodesmosomes. PMID: 21628128
- This study identified mRNA transcripts from three genes, CDSN, LOR, and KRT9, exhibiting strong overexpression in skin samples compared to samples from forensic body fluids, making them suitable markers for skin identification. PMID: 21221983
- This study on consanguinity in a large family points to a homozygous nonsense mutation in the gene encoding corneodesmosin. PMID: 21134591
- The distribution of corneodesmosin on the surface of the stratum corneum was mapped at the nanoscale using atomic force microscopy. PMID: 21182673
- Data identifies hypotrichosis simplex of the scalp as a human amyloidosis related to the aggregation of natively unfolded (mut)CDSN polypeptides into amyloid fibrils. PMID: 20448140
- The lack of corneodesmosin causes an epidermal barrier defect, which is thought to contribute to the predisposition to atopic diseases. PMID: 20691404
- This study identified nonsense mutations in the CDSN gene (encoding corneodesmosin) in three families suffering from hypotrichosis simplex of the scalp. PMID: 12754508
- Non-glycosylated CDSN is capable of spontaneously forming large homo-oligomers in vitro, and the N-terminal glycine loop domain is essential for the formation of these macromolecular complexes. PMID: 15086562
- Association analyses indicate that haplotypes carrying CDSN*971T map to an RNA stability motif and contribute to psoriasis susceptibility across various ethnic groups. PMID: 15333584
- The phenotype of Netherton syndrome is a consequence of desmosomal fragility associated with premature proteolysis of corneodesmosin. PMID: 15466487
