CEP120 Antibody

Antibody Characteristics

CEP120 antibodies are polyclonal reagents primarily generated in rabbits, targeting human and mouse CEP120. Key features include:

Experimental Applications and Dilutions

Validated protocols and recommended dilutions for CEP120 antibodies:

Table 1: Application-Specific Dilutions

Research Findings on CEP120 Biology

CEP120 antibodies have been instrumental in uncovering the protein’s roles in cellular processes:

• Centrosome Homeostasis: Cep120 depletion in mouse embryonic fibroblasts disrupts procentriole assembly and centrosome maturation, while overexpression inhibits pericentrin accumulation at centrosomes .

• Neural Development: CEP120 regulates centrosome-mediated nuclear migration in neural progenitors, critical for cortical development .

• Centriole Elongation: Collaborates with SPICE1 and CPAP to recruit CEP135 and distal microtubule-capping proteins during centriole duplication .

Clinical and Disease Relevance

CEP120 dysregulation is implicated in cancer progression:

Table 2: CEP120 in Gastric Cancer (GC)

Protocols and Best Practices

• Antigen Retrieval: For IHC, use TE buffer (pH 9.0) or citrate buffer (pH 6.0) .

• Cell Cycle Synchronization: Serum starvation (48 hr in low-serum medium) for studying quiescent cells .

• Validation Controls: Include Ki-67 staining to confirm cell proliferation status in functional assays .