cex-2 Antibody

Target Protein Overview

Carboxylesterase 2 (CES2) is a serine hydrolase encoded by the CES2 gene (Accession #O00748) and expressed in tissues such as the liver and intestines. It catalyzes the hydrolysis of ester- and amide-bond-containing compounds, including prodrugs like irinotecan, into active metabolites. CES2's structure spans Gln27-Leu559, with a molecular weight of ~60–65 kDa under reducing conditions .

Antibody Characteristics

CES2 antibodies are typically monoclonal or polyclonal reagents optimized for specificity and reproducibility:

Western Blotting

• Detects CES2 in human liver tissue and cancer cell lines (e.g., HepG2 hepatocellular carcinoma) .

• Protocol: 2 µg/mL primary antibody concentration with HRP-conjugated secondary antibodies .

Cellular Localization

• Immunocytochemistry confirms cytoplasmic expression in HepG2 cells using NorthernLights™ 557-conjugated secondary antibodies .

Therapeutic Monitoring

• CES2 antibodies aid in studying enzyme-drug interactions, particularly in prodrug activation pathways .

Cation Exchange Chromatography-Mass Spectrometry (CEX-MS)

• Resolves charge variants (e.g., deamidation, glycation) in monoclonal antibodies with pH gradients .

• Automated workflows enable proteoform quantification (e.g., Adalimumab variants) .

Charge Variant Analysis

• CEX-UV-MS identifies mispaired light chains in bispecific antibodies by comparing theoretical vs. observed pI values .