CHP Human
Description
Table 1: Key Protein Overlap Between iCSF and Human CSF
Transcriptomic Validation
Single-cell RNA sequencing (scRNA-seq) confirms organoids replicate developmental trajectories:
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Cell-type composition: 92% similarity to human embryonic ChP epithelium (vs. 78% to mouse) .
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Maturation markers: Transthyretin (TTR) and CLIC6 expression increases 3.8-fold between days 28–68 .
Table 2: Developmental Gene Expression Dynamics
| Gene | Embryonic ChP (E16.5) | Organoid (Day 58) | Organoid (Day 68) |
|---|---|---|---|
| TTR | 12.1 TPM | 8.4 TPM | 32.7 TPM |
| CLIC6 | 9.8 TPM | 7.2 TPM | 28.9 TPM |
| AQP1 | 15.3 TPM | 14.1 TPM | 16.5 TPM |
| TPM = transcripts per million . |
Applications in Drug Development
ChP organoids predict neurotoxicity and drug permeability:
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BIA 10-2474: Accumulation in iCSF due to impaired efflux correlates with clinical neurotoxicity .
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CNS drug screening: Organoids correctly classify 22/24 compounds for blood-CSF barrier penetration (91.7% accuracy) .
Limitations and Innovations
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Vascular absence: Organoids lack blood vessels, excluding blood-derived CSF components (e.g., hemoglobin) .
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Aging models: Proteomic shifts between days 58–68 mimic human CSF aging patterns (ρ=0.79, p<0.001) .
Future Directions
Product Specs
MGSSHHHHHH SSGLVPRGSH MMGSRASTLL RDEELEEIKK ETGFSHSQIT RLYSRFTSLD KGENGTLSRE DFQRIPELAI NPLGDRIINA FFPEGEDQVN FRGFMRTLAH FRPIEDNEKS KDVNGPEPLN SRSNKLHFAF RLYDLDKDEK ISRDELLQVL RMMVGVNISD EQLGSIADRT IQEADQDGDS AISFTEFVKV LEKVDVEQKM SIRFLH.
Product Science Overview
Calcium Binding Protein P22 is a full-length protein consisting of 195 amino acids. It is expressed in Escherichia coli and has a purity of over 95%, making it suitable for various applications such as SDS-PAGE and mass spectrometry (MS) . The protein includes an N-terminal His-tag, which facilitates its purification and detection in experimental settings .
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Regulation of Vesicular Trafficking: Calcium Binding Protein P22 is involved in the regulation of vesicular trafficking, particularly in the constitutive exocytic membrane traffic. It mediates the association between microtubules and membrane-bound organelles of the endoplasmic reticulum and Golgi apparatus .
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Plasma Membrane Na+/H+ Exchanger: This protein functions as an integral cofactor in cell pH regulation by controlling the activity of the plasma membrane-type Na+/H+ exchanger (NHE). It affects the pH sensitivity of SLC9A1/NHE1 by increasing its sensitivity at acidic pH, thereby stabilizing and localizing SLC9A1/NHE1 at the plasma membrane .
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Gene Transcription: Calcium Binding Protein P22 also plays a role in gene transcription. It acts as an inhibitor of ribosomal RNA transcription by repressing the nucleolar UBF1 transcriptional activity. This inhibition may involve sequestering UBF1 in the nucleoplasm and limiting its translocation to the nucleolus .
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Inhibition of Calcineurin Activity: The protein has sequence similarity to calcineurin B and is known to be an endogenous inhibitor of calcineurin activity. This inhibition is crucial for maintaining cellular homeostasis and regulating various signaling pathways .
