Customize CHX23 Antibody

Description

Potential Query Misinterpretation

The term "CHX23" may stem from typographical errors or confusion with established antibodies. Below are the closest matches identified:

Candidate Antibody	Relevance	Source
CHCHD3 Antibody	Targets coiled-coil-helix domain-containing protein 3 (CHCHD3), a mitochondrial regulator. Validated in WB, IHC, and IF/ICC applications.
ChemR23 Antibody (MAB362)	Detects ChemR23/CMKLR1, a chemokine receptor involved in neutrophil modulation and inflammation. Validated in flow cytometry and immunofluorescence.
Chd2 Antibody (MABE873)	Monoclonal antibody against chromatin remodeler CHD2. Validated for WB, IP, ICC, and ChIP.

Terminology Breakdown

"CHX": Common prefix for chemical compounds (e.g., CHX denotes cycloheximide) but not antibody nomenclature.
"23": Often denotes clone numbers (e.g., clone 8H3 in Chd2 Antibody ).

Discrepancies in Available Data

No antibodies with the "CHX23" designation appear in:
- Commercial catalogs (Proteintech, R&D Systems, Merck Millipore) .
- Clinical-stage therapeutics (e.g., chimeric/humanized antibodies in ).
- Research literature on camelid sdAbs or monoclonal antibodies .

Recommended Alternatives for Further Investigation

If seeking antibodies with similar functional roles, consider:

Table 1: Antibodies Targeting Mitochondrial or Inflammatory Proteins

Antibody	Target	Applications	Key Features
CHCHD3 (25625-1-AP)	CHCHD3	WB, IHC, IF/ICC	Polyclonal, validated in human/mouse samples
ChemR23 (MAB362)	CMKLR1	Flow cytometry, calcium flux assays	Detects surface expression on neutrophils
Chd2 (MABE873)	CHD2	ChIP, ICC	Chromatin remodeling studies

Hypothetical Scenario: CHX23 Antibody Development

If "CHX23" refers to a novel, uncharacterized antibody, standard validation steps would include:

Validation Parameters

Parameter	Method	Example from Literature
Specificity	SDS-PAGE, mass spectrometry	Batch consistency in anti-Dsg3 antibody 2G4
Functionality	ELISA, flow cytometry	ChemR23 detection in neutrophil activation
Pathogenicity	Dissociation assays	AK23-induced keratinocyte fragmentation

Product Specs

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Lead Time

Made-to-order (14-16 weeks)

Synonyms

CHX23 antibody; At1g05580 antibody; F3F20.2Cation/H(+) antiporter 23 antibody; chloroplastic antibody; Protein CATION/H+ EXCHANGER 23 antibody; AtCHX23 antibody

Target Names

CHX23

Uniprot No.

Q8VYD4

Target Background

Function

CHX23 Antibody targets a protein that functions as a K(+)/H(+) or Na(+)/H(+) antiporter within the chloroplast envelope. This protein plays a crucial role in maintaining pH balance and facilitating chloroplast development. It exhibits a preference for transporting monovalent cations, particularly Cs(+), K(+), and Rb(+) over Na(+) or Li(+). Additionally, it is essential for pollen tube guidance, although it does not affect normal pollen development. The antibody may also be involved in the development or function of the female gametophyte.

Database Links

KEGG: ath:AT1G05580

STRING: 3702.AT1G05580.1

UniGene: At.42390

Protein Families

Monovalent cation:proton antiporter 2 (CPA2) transporter (TC 2.A.37) family, CHX (TC 2.A.37.4) subfamily

Subcellular Location

Plastid, chloroplast membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Note=PubMed:15220473 shows a localization in chloroplasts while PubMed:21239645 shows a localization in ER.

Tissue Specificity

Specifically expressed in flower buds and pollen. Expressed in leaves, roots and stems.

Q&A

FAQs for CHX23 Antibody in Academic Research

Advanced Research Questions

How can researchers resolve discrepancies between CHX23 Antibody data from ELISA vs. flow cytometry?
- Investigate epitope accessibility: Conformational epitopes may be masked in flow cytometry due to fixation .
- Quantify avidity effects using surface plasmon resonance (SPR) to measure binding kinetics .
What computational strategies improve CHX23 Antibody targeting in complex biological matrices?
- Apply binding energy modeling (e.g., RosettaAntibody) to predict paratope-epitope interactions .
- Use molecular dynamics simulations to assess stability in physiological pH/salt conditions .
How do post-translational modifications affect CHX23 Antibody recognition of its antigen?
- Perform phosphorylation/glycosylation profiling of antigen-expressing cells via mass spectrometry .
- Compare antibody binding using phosphatase-treated vs. untreated lysates in Western blots .

Data Interpretation & Troubleshooting

What methodologies validate CHX23 Antibody’s role in observed phenotypic changes?
- Combine CRISPR interference (CRISPRi) with antibody-based protein depletion for orthogonal validation .
- Analyze dose-response curves (e.g., EC₅₀ calculations) across biological replicates .
How should researchers address batch-to-batch variability in CHX23 Antibody performance?
- Standardize using internal reference standards (e.g., aliquots from a validated master batch) .
- Implement inter-laboratory proficiency testing with shared positive/negative samples .

Methodological Guidance for Common Scenarios
Scenario: Inconsistent CHX24A1 upregulation in LRH-1 pathway studies using CHX23 Antibody .

Solution:
- Verify hLRH-1 expression levels via qPCR/Western blot (Fig. 7A) .
- Test antibody dependency using LRH-1 knockdown models (Fig. 8) .
- Screen for ligand-binding pocket mutations that alter antibody-antigen interactions (Fig. 11) .

Scenario: Cross-reactivity with unrelated phosphoproteins in kinase assays.

Solution:
- Perform immunodepletion assays to isolate target-specific signals .
- Utilize cross-linking mass spectrometry to map off-target binding sites .

Key Validation Metrics

Parameter	Acceptable Threshold	Assay Type
Lot Consistency	CV < 15%	ELISA/Flow Cytometry
Thermal Stability	≥80% activity at 4°C	Accelerated Aging
Cross-Reactivity	≤5% vs. homologs	Peptide Array

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CHX23 Antibody