CHX28 Antibody
Description
Introduction to CHX28 Antibody
The CHX28 Antibody is a specialized immunological reagent designed for research applications targeting the CHX28 protein in Arabidopsis thaliana (Mouse-ear cress). This antibody is part of a broader catalog of plant biology research tools, facilitating studies on ion transport mechanisms, stress responses, and developmental processes in model plants .
Research Applications
While direct studies on CHX28 are not explicitly detailed in the provided sources, its homologs in the CHX (Cation/H⁺ Exchanger) family are implicated in:
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Ion Homeostasis: Regulation of potassium and sodium transport under abiotic stress conditions .
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Cellular Localization: Predominantly associated with endomembrane systems, including vacuoles and Golgi .
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Stress Response: Role in salinity and drought tolerance, inferred from functional studies on related CHX proteins .
Validation and Characterization
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Western Blotting: Confirm target specificity using Arabidopsis lysates.
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Immunohistochemistry (IHC): Localize CHX28 in plant tissue sections.
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Knockout Validation: Compare signal in wild-type vs. CHX28-knockout mutants.
Note: Users should optimize protocols for their experimental systems.
Comparative Analysis with Related Antibodies
The CHX28 Antibody’s performance can be contextualized against other plant-targeting antibodies (e.g., CER3, CML8) from the same catalog :
| Antibody Target | UniProt ID | Key Function | Validation Status (Reported) |
|---|---|---|---|
| CHX28 | Q8L709 | Cation transport | Limited data |
| CER3 | Q8H1Z0 | Cuticular wax biosynthesis | IHC, WB validated |
| CML8 | O23320 | Calcium signaling | ELISA, WB validated |
Future Research Directions
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Functional Studies: Elucidate CHX28’s role in ion transport using CRISPR-edited Arabidopsis lines.
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Cross-Reactivity Profiling: Assess specificity across plant species (e.g., Oryza sativa).
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Structural Epitope Mapping: Define binding regions using peptide microarrays .
Access and Availability
The CHX28 Antibody is commercially available through CUSABIO (Product Code: CSB-PA813151XA01DOA) . Researchers are advised to:
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Validate lot-specific performance via technical support.
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Refer to UniProt entry Q8L709 for sequence alignment and epitope prediction.
Product Specs
Constituents: 50% Glycerol, 0.01M Phosphate Buffered Saline (PBS), pH 7.4
Target Background
Q&A
Given the lack of specific information on "CHX28 Antibody" in the provided search results, I will create a general FAQ for researchers focusing on antibody-related research, particularly in the context of experimental design and data analysis. This will include advanced research questions and methodological answers relevant to academic research scenarios.
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To evaluate the efficacy of a novel antibody, you should:
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Select Relevant Cell Lines: Choose cell lines that express the target antigen.
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Optimize Antibody Concentration: Perform dose-response experiments to determine the optimal concentration.
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Control Groups: Include negative controls (e.g., untreated cells) and positive controls (e.g., known effective antibodies).
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Assessment Methods: Use techniques like flow cytometry, Western blotting, or ELISA to measure antibody binding and functional effects.
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Re-evaluate Experimental Conditions: Ensure that all experiments were conducted under identical conditions (e.g., same cell line, media, temperature).
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Statistical Analysis: Use appropriate statistical tests to compare results and determine if differences are significant.
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Replication: Repeat experiments multiple times to confirm findings.
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Consider Alternative Explanations: Look for potential biases or confounding factors that might explain discrepancies.
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Epitope mapping is crucial for understanding how antibodies interact with their antigens. Common methods include:
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X-ray Crystallography: Provides high-resolution structural information about the antibody-antigen complex.
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Mutagenesis Studies: Systematically mutate residues in the antigen to identify critical binding sites.
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Peptide Array Analysis: Uses arrays of peptides to identify specific sequences recognized by the antibody.
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Site-Directed Mutagenesis: Introduce targeted mutations into the antibody's variable regions to enhance binding.
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Phage Display Technology: Utilize libraries of antibodies to select variants with improved properties.
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Computational Modeling: Use structural models to predict and design mutations that enhance affinity or specificity.
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Model Selection: Choose appropriate animal models that mimic human disease conditions.
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Dose and Route of Administration: Optimize dosing regimens and administration routes for efficacy and safety.
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Safety Monitoring: Monitor for potential side effects, such as cytokine release syndrome or immune reactions.
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Pharmacokinetics and Pharmacodynamics: Study how the antibody is absorbed, distributed, metabolized, and excreted, and how it affects biological processes.
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Clear Methodology: Clearly describe experimental methods and data analysis techniques.
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Data Visualization: Use appropriate graphs and tables to present findings effectively.
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Statistical Significance: Highlight statistically significant results and discuss their implications.
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Discussion of Limitations: Address potential limitations and areas for future research.
Example Data Table: Antibody Efficacy Comparison
| Antibody | Concentration (μg/mL) | Cell Line | Binding Efficiency (%) |
|---|---|---|---|
| A | 10 | HEK293 | 80 |
| B | 5 | MCF7 | 60 |
| C | 20 | Jurkat | 90 |
This table illustrates how different antibodies (A, B, C) perform at various concentrations across different cell lines, providing a basis for comparison and further optimization.
