cid1 Antibody
Description
cid1 in Fission Yeast Biology
The cid1 gene in Schizosaccharomyces pombe (fission yeast) encodes a cytoplasmic poly(A) polymerase involved in RNA metabolism and S-M checkpoint control during DNA replication stress. Key findings include:
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Function: Cid1 polyadenylates RNA substrates, influencing mRNA stability and translation. Unlike canonical poly(A) polymerases, Cid1 operates in the cytoplasm and targets specific RNA subsets .
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Checkpoint Role: Cid1 supports cell survival under replication stress (e.g., hydroxyurea exposure) by delaying mitosis until DNA synthesis resumes. Deletion of cid1 causes sensitivity to replication inhibitors and checkpoint failure .
| Key Properties of cid1 Protein |
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| Molecular Weight |
| Activity |
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| Substrates |
| Mutant Phenotype |
No antibodies targeting the fission yeast cid1 protein are described in the provided sources.
Potential Misinterpretation: CD1d Antibodies
If the query refers to CD1d (a human MHC-like antigen-presenting molecule), multiple antibodies are documented:
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CD1d-Specific VHH: Camelid-derived single-domain antibodies targeting CD1d show therapeutic potential:
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Functional Applications:
| Anti-CD1d VHH Clones | Mechanism | Effect |
|---|---|---|
| VHH 17 | Binds CD1d, triggers apoptosis | Reduces viability of CD1d+ cancer cells |
| VHH 22 | Blocks CD1d–iNKT TCR interaction | Suppresses cytokine production (IFN-γ, TNF-α) |
CIDEC Antibody
A separate entry for CIDEC antibody (Proteintech 12287-1-AP) was identified, though CIDEC is unrelated to cid1:
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- Research demonstrates that a hinge region (N164-N165) within the Cid1 terminal uridylyltransferase is critical for its catalytic activity. PMID: 25712096
- Structural analysis of Cid1 in complex with the non-hydrolyzable nucleotide UMPNPP and its minimal pseudo-product ApU has revealed new key residues involved in substrate/product recognition. PMID: 24322298
- Studies have shown that Cid1 can accommodate all four tested ribonucleotide triphosphates (NTPs) in its active site. However, differences in recognition suggest that UTP is the preferred NTP. PMID: 22885303
- Crystal structures of Cid1 in apo conformers and bound to UTP demonstrate that a single histidine residue, conserved across mammalian Cid1 orthologs, is responsible for discriminating between UTP and ATP. PMID: 22751018
- Cid1 exhibits RNA binding properties, a feature with significant implications for miRNAs, histone mRNAs, and, more broadly, cellular RNA degradation. PMID: 22608966
- Research describes the biochemical activity of fission yeast Schizosaccharomyces pombe Cid1, a putative cytoplasmic poly(A) polymerase implicated in cell cycle checkpoint controls. PMID: 17353264
- The Schizosaccharomyces pombe cytoplasmic protein Cid1 acts as a poly(U) polymerase (PUP). Polyadenylated actin mRNA, a target of this activity, undergoes uridylation upon arrest in S phase, and likely represents one of many Cid1 targets. PMID: 18371314
KEGG: spo:SPAC19D5.03
STRING: 4896.SPAC19D5.03.1
