zgc:153142 Antibody
Description
Target and Origin
The zgc:153142 Antibody is a polyclonal antibody raised against the protein product of the zgc:153142 gene in zebrafish. Polyclonal antibodies are generated by immunizing animals (typically rabbits or mice) with the target antigen, which triggers a diverse immune response and the production of multiple antibody types. This diversity enhances the antibody’s ability to bind various epitopes on the target protein .
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Target Protein: The zgc:153142 gene encodes a protein with the UniProt ID Q08BN1, though its specific function remains under investigation. Zebrafish genes with the "zgc" prefix are often novel or uncharacterized genes identified through genome sequencing initiatives .
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Species Specificity: The antibody is validated for use in Danio rerio (zebrafish), a model organism widely used in developmental biology and disease studies due to its transparent embryos and conserved genetic pathways .
Structure and Function
Like all antibodies, the zgc:153142 Antibody consists of two heavy chains and two light chains, forming a "Y" shape. Its structure includes:
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Variable Regions (Fab): These regions at the tips of the antibody determine specificity for the target antigen. The complementarity-determining regions (CDRs) within the variable regions form loops that interact with the antigen’s epitope .
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Constant Regions (Fc): These regions mediate effector functions, such as binding to immune cells or complement proteins, though this antibody is likely used for detection rather than therapeutic purposes .
The antibody’s production involves enzymatic digestion and purification steps to ensure high purity and specificity. Its isotype and epitope binding characteristics are not explicitly disclosed in the available data, but polyclonal antibodies typically recognize multiple epitopes on the target protein .
Research Applications
While specific studies using the zgc:153142 Antibody are not documented in the provided sources, its design aligns with common applications of zebrafish antibodies in:
The antibody’s utility could extend to investigating conserved genetic mechanisms between zebrafish and humans, as zebrafish are often used to model human diseases such as cancer and neurodegenerative disorders .
Research Gaps and Future Directions
The zgc:153142 Antibody’s role in research remains underexplored, as no peer-reviewed studies explicitly cite its use. Future investigations could focus on:
Product Specs
Constituents: 50% Glycerol, 0.01M Phosphate Buffered Saline (PBS), pH 7.4
Q&A
Basic Research Questions
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What is zgc:153142 and what is its significance in zebrafish research?
zgc:153142 is a protein encoded by a zebrafish (Danio rerio) gene with the Entrez Gene ID 768197. The protein has a UniProt number of Q08BN1. While the specific function of this protein remains under investigation, it is studied in zebrafish as a model organism for understanding vertebrate development and disease mechanisms.
Research using zgc:153142 antibody allows scientists to:
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Track protein expression across developmental stages
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Identify tissue-specific localization patterns
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Investigate potential roles in signaling pathways
Like other zebrafish gene products (e.g., zgc:153631 and zgc:153352), zgc:153142 serves as an important marker in developmental biology studies .
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What types of zgc:153142 antibodies are currently available for research applications?
Currently, the primary commercially available zgc:153142 antibody is a rabbit polyclonal antibody. Key characteristics include:
Characteristic Specification Article Number CSB-PA600564XA01DIL-0.2 Clonality Polyclonal Host Rabbit Immunogen Recombinant Danio rerio zgc:153142 protein Isotype IgG Size 0.2mg Conjugation Unconjugated Applications ELISA, Western Blot (WB) Species Reactivity Zebrafish The antibody components typically include:
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What are the optimal storage conditions for zgc:153142 antibody?
For maximum stability and retention of activity, zgc:153142 antibody should be stored according to these guidelines:
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Avoid repeated freeze-thaw cycles which can cause protein denaturation and reduced activity
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For working aliquots, store at 4°C for up to one week
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When diluting from stock, use appropriate buffers (e.g., PBS with 0.1% BSA) to maintain protein stability
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Document lot numbers and date of reconstitution for experimental reproducibility
Proper storage is critical as research has shown that approximately 50% of antibody activity can be lost through improper handling and storage conditions .
