At1g74320 Antibody

Definition and Target Identification

The At1g74320 Antibody is a polyclonal antibody designed to target the protein encoded by the At1g74320 gene in Arabidopsis thaliana (Mouse-ear cress). This gene locus corresponds to a hypothetical protein with UniProt identifier Q8L518, though its precise biological function remains uncharacterized in public databases . The antibody is primarily used in plant molecular biology research to study gene expression, protein localization, and functional assays in Arabidopsis models.

Protein Localization Studies

The antibody has been employed to investigate subcellular localization, with preliminary data suggesting chloroplast stromal signals in transgenic Arabidopsis lines .

Stress Response Profiling

Unpublished studies cited by manufacturers indicate upregulated At1g74320 protein levels under drought and high-salinity conditions, implicating it in abiotic stress adaptation .

Interaction Networks

Co-immunoprecipitation (Co-IP) experiments using this antibody have identified putative binding partners, including:

• CKL1 (Clathrin heavy chain homolog)

• CIPK6 (CBL-interacting serine/threonine-protein kinase 6)

Challenges and Limitations

• Specificity Issues: Cross-reactivity with unrelated chloroplast proteins observed at concentrations >1:500 .

• Functional Data Gap: No peer-reviewed publications directly link At1g74320 to specific pathways.

• Epitope Mapping: The immunogen peptide (exact sequence undisclosed) may target variable regions, limiting reproducibility .

Future Research Directions

1. CRISPR Knockout Models: Establish phenotypic changes in At1g74320 mutants under stress conditions.

2. Structural Biology: Resolve 3D conformation via cryo-EM using antibody-assisted purification.

3. Omics Integration: Correlate protein abundance with RNA-seq and metabolomics datasets.