At5g06830 Antibody
Description
Identification and Target Specificity
At5g02830 Antibody is a rabbit polyclonal antibody developed to target the Arabidopsis thaliana protein encoded by the At5g02830 gene. This antibody is commercialized by Biorbyt (catalog number orb793981) and is validated for use in:
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ELISA
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Immunoassay
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Western Blot (WB)
Key Specifications (adapted from ):
| Parameter | Detail |
|---|---|
| Host | Rabbit |
| Immunogen | Recombinant Arabidopsis thaliana At5g02830 protein |
| Isotype | IgG |
| Reactivity | Arabidopsis thaliana |
| Applications | EIA, Immunoassay, ELISA, Western Blot |
| Conjugation | Unconjugated |
| Storage | -20°C or -80°C (avoid repeated freeze-thaw cycles) |
Antibody Design and Mechanism
The At5g02830 Antibody binds to epitopes within the At5g02830 protein, enabling detection in plant tissues. Polyclonal antibodies like this one recognize multiple epitopes, enhancing sensitivity in techniques such as Western blotting. For example, in plant protein extraction workflows, the antibody is used to identify At5g02830 in lysates or membrane fractions.
Challenges and Considerations
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Cross-reactivity: Polyclonal antibodies may bind non-specifically to homologous proteins in other species, requiring validation.
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Quantitative Limitations: Absence of data on antibody affinity (Kd values) or epitope mapping complicates precise quantification.
Critical Gaps and Future Directions
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Lack of Epitope Mapping: No studies detail the specific regions of At5g02830 targeted by this antibody, limiting reproducibility.
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Cross-Species Reactivity: Uncertainty exists regarding binding to orthologs in other plant species (e.g., crops like maize or rice).
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Functional Validation: Absence of data on antibody-mediated protein neutralization or interference in cellular processes.
