cka-2 Antibody
Description
Introduction to CK2α Antibody
The CK2α antibody is a research tool designed to detect the catalytic subunit α of casein kinase 2 (CK2), a serine/threonine kinase involved in critical cellular processes such as cell cycle regulation, apoptosis, and immune signaling. CK2α (CSNK2A1) forms tetrameric complexes with regulatory β subunits (CK2β) to phosphorylate over 500 substrates, influencing tumor progression, inflammation, and immune evasion . The antibody is essential for studying CK2α’s role in normal physiology and pathologies like cancer, autoimmune diseases, and fungal infections.
Applications and Techniques
CK2α antibodies are validated for diverse applications:
Role in Cancer and Immune Regulation
-
PD-L1 Stabilization: CK2α phosphorylates PD-L1 at Thr285/Thr290, preventing its degradation and promoting immune evasion in tumors. Inhibition of CK2 reduces PD-L1 levels, enhancing T-cell activity .
-
Tumor Microenvironment: CK2α antibodies revealed that CK2 inhibitors (e.g., CX-4945) decrease myeloid-derived suppressor cells (MDSCs) and tumor-associated macrophages (TAMs), improving anti-tumor responses .
Fungal Pathogenesis
In Cryptococcus neoformans, CK2α (Cka1) regulates stress response, virulence factor production (e.g., melanin, capsule), and antifungal drug resistance. Antibodies targeting Cka1 could guide antifungal drug development .
Common CK2α Antibodies
Validation Data
-
Western Blot: Detects a 42–47 kDa band in HeLa, NIH-3T3, and HAP1 cell lysates. KO cell lines confirm specificity .
-
Immunofluorescence: Localizes CK2α to nuclei in HEK293 and MCF-7 cells .
-
IHC: Identifies nuclear CK2α expression in human brain and colon cancer tissues .
Signaling Pathways
Therapeutic Implications
Product Specs
Constituents: 50% Glycerol, 0.01M Phosphate Buffered Saline (PBS), pH 7.4
