CKB Antibody

Creatine Kinase Brain, Mouse Anti Human
Shipped with Ice Packs
In Stock
Cat. No.
BT15062
Synonyms
Creatine kinase B-type, EC 2.7.3.2, Creatine kinase B chain, B-CK, CKB, CKBB, CKBBI.
Appearance
Sterile filtered colorless solution.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Quick Inquiry

Description

Key Applications and Dilutions

ApplicationRecommended DilutionValidated Samples
Western Blot (WB)1:500–1:50,000 HEK-293 cells, mouse brain, human tissues
Immunohistochemistry1:50–1:2,000 Mouse brain, human skeletal muscle
Immunoprecipitation0.5–4.0 µg Mouse brain lysates
Flow Cytometry1:50–1:100 Human cervix carcinoma cells

Research Findings

  • Cancer Metastasis: CKB suppresses epithelial-mesenchymal transition (EMT) in prostate cancer by inhibiting AKT activation . Downregulation of CKB correlates with poor prognosis in solid tumors .

  • Metabolic Regulation: In breast cancer, hypoxia-inducible factor (HIF)-dependent CKB expression promotes metastasis, while cyclocreatine therapy inhibits invasion .

  • Diagnostic Utility: Immunohistochemical staining using anti-CKB antibodies (e.g., ab108388) revealed significantly lower CKB levels in tumors vs. normal tissues (p < 0.001) .

4. Commercial Availability
Multiple vendors offer CKB antibodies with distinct properties:

ProductHost/ClonalityReactivityApplicationsKey Features
CAB4907 Rabbit/MonoclonalHuman, MouseWB, IHC-P, ELISATargets N-terminal (1–100 aa) of CKB
15137-1-AP Rabbit/PolyclonalHuman, MouseWB, IP, IHCRecognizes both CKB and CKM isoforms
CPTC-CKB-3 Mouse/MonoclonalHumanWB, ELISAValidated for recombinant and endogenous CKB
A01695 Rabbit/PolyclonalHuman, MouseWB, IHC, Flow CytometryBSA-free options available

Clinical and Mechanistic Relevance

  • Prostate Cancer: CKB sequesters AKT, blocking mTORC2-mediated activation and reducing metastasis . A C-terminal 84-amino-acid fragment of CKB is critical for this inhibition .

  • Breast Cancer: HIF-1α upregulates CKB under hypoxia, enhancing ATP production for invadopodia formation . Cyclocreatine, a CK inhibitor, reduces metastasis and synergizes with chemotherapy .

  • Neurological Disorders: CKB is highly expressed in brain tissue, with implications for neurodegenerative diseases .

Technical Considerations

  • Storage: Stable at -20°C in PBS with 0.02% sodium azide and 50% glycerol .

  • Cross-Reactivity: Most antibodies are specific to CKB, though 15137-1-AP cross-reacts with CKM .

  • Validation: Antibodies are tested via CRISPR/Cas9 KO, siRNA knockdown, and recombinant protein controls .

Product Specs

Introduction
Creatine Kinase BB, also known as CKB, is an enzyme that plays a vital role in cellular energy regulation, particularly in tissues with high energy demands like the brain. It facilitates the reversible transfer of phosphate between ATP, the primary energy currency of cells, and creatine phosphate, a molecule that acts as an energy reservoir. CKB primarily functions as a homodimer in the brain and other tissues, but it can also form heterodimers with similar muscle isozymes in the heart. This enzyme is classified under the ATP:guanido phosphotransferase protein family. Interestingly, a non-functional copy of the CKB gene, termed a pseudogene, has also been identified.
Physical Appearance
The product is a clear and colorless solution that has been sterilized by filtration.
Formulation
This product is provided at a concentration of 1mg/ml and is prepared in a solution containing phosphate buffered saline (PBS) at a pH of 7.4, 10% glycerol, and 0.02% sodium azide as a preservative.
Storage Procedures
For short-term storage of up to one month, the product should be kept at 4°C. For extended storage periods, it is recommended to store the product at -20°C. To maintain product integrity and activity, it is crucial to avoid repeated freeze-thaw cycles.
Stability / Shelf Life
The product has a shelf life of 12 months when stored properly at -20°C. At 4°C, the product is stable for 1 month.
Applications
The CKB antibody has undergone rigorous testing in various applications, including ELISA, Western blot analysis, Flow cytometry, and Immunocytochemistry/Immunofluorescence (ICC/IF), to ensure its specificity and reactivity. However, it is essential to note that optimal working dilutions may vary depending on the specific application and experimental conditions. Therefore, users are advised to perform their own titrations to determine the most effective antibody concentration for their intended use.
Synonyms
Creatine kinase B-type, EC 2.7.3.2, Creatine kinase B chain, B-CK, CKB, CKBB, CKBBI.
Purification Method
CKB antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
PAT8E4AT.
Immunogen
Anti-human CKB mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human CKB amino acids 1-381 purified from E. coli.
Ig Subclass
Mouse IgG2a heavy chain and k light chain.

Product Science Overview

Creatine Kinase Brain (CK-BB)

CK-BB, also known as brain creatine kinase, is a cytoplasmic enzyme involved in energy metabolism. It is encoded by the CKB gene and is a member of the ATP:guanido phosphotransferase protein family. CK-BB acts as a homodimer in the brain and other tissues, and as a heterodimer with a similar muscle isozyme in the heart . The enzyme is crucial for maintaining energy homeostasis in cells with high and fluctuating energy demands, such as neurons .

Mouse Anti-Human CK-BB Antibody

Mouse anti-human CK-BB antibodies are monoclonal antibodies produced by immunizing mice with human CK-BB protein. These antibodies are highly specific and are used in various research applications, including Western blotting, immunohistochemistry, and ELISA. They are valuable tools for studying the expression and function of CK-BB in different tissues and under various physiological and pathological conditions .

Applications and Importance
  1. Research: Mouse anti-human CK-BB antibodies are extensively used in research to investigate the role of CK-BB in energy metabolism, neurodegenerative diseases, and other conditions affecting the brain and other tissues .
  2. Diagnostics: These antibodies can be used in diagnostic assays to detect CK-BB levels in biological samples, which can provide insights into various diseases and conditions .
  3. Therapeutics: Understanding the function and regulation of CK-BB can lead to the development of therapeutic strategies for diseases involving energy metabolism dysregulation .

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

CKB Human His
Creatine Kinase Brain Human Recombinant, His Tag
Cat. No.
BT30946
Source
Escherichia Coli.
CKB Human, Pichia
Creatine Kinase Brain Human Recombinant, Pichia
Cat. No.
BT30966
Source
Pichia Pastoris.
CKB Human
Creatine Kinase Brain Human Recombinant
Cat. No.
BT9408
Source
E.coli.
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.