CKMT1A Antibody

Creatine Kinase, Mitochondrial 1A, Mouse Anti Human
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Cat. No.
BT15140
Synonyms
Creatine kinase mitochondrial 1A, creatine kinase mitochondrial 1 (ubiquitous), creatine kinase U-type mitochondrial, Acidic-type mitochondrial creatine kinase, Ubiquitous mitochondrial creatine kinase, CKMT1, U-MtCK, mia-CK, EC 2.7.3, EC 2.7.3.2.
Appearance
Sterile filtered colorless solution.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Introduction to CKMT1A Antibody

CKMT1A antibody is a research tool designed to detect and quantify creatine kinase mitochondrial 1A (CKMT1A), a key enzyme in mitochondrial energy metabolism. This antibody is critical for studying CKMT1A's role in cellular energetics, cancer progression, and metabolic disorders. Below is a detailed analysis of its applications, research findings, and validation data.

Structure and Function of CKMT1A

CKMT1A is encoded by the CKMT1A gene located on chromosome 15q15.3, consisting of 11 exons. The protein comprises 417 amino acids (47 kDa) and exists as homodimers or octamers in the mitochondrial intermembrane space . Its primary function involves catalyzing the reversible transfer of γ-phosphate from ATP to creatine, generating phosphocreatine (PCr), a critical energy shuttle molecule .

CharacteristicsDetails
Gene LocationChromosome 15q15.3
Protein Structure417 amino acids; 47 kDa; forms dimers/octamers
Enzymatic ActivityATP → ADP + PCr; energy shuttle between mitochondria and cytosol
Tissue ExpressionUbiquitous, with high levels in heart, brain, and muscle

Applications of CKMT1A Antibody

CKMT1A antibodies are validated for multiple techniques, enabling researchers to study its expression and localization in diverse biological contexts.

Tested Applications and Reactivity

ApplicationReactivitySample Sources
Western BlotHuman, Mouse, RatHeart, colon, testis, HEK-293, A549, and NSCLC cells
ImmunohistochemistryHumanColon cancer, breast cancer (TE buffer pH 9.0 recommended)
ImmunoprecipitationMouseHeart tissue lysates
ELISAHuman, MouseSerum and tissue lysates

Product Specs

Introduction
CKMT1A, a member of the creatine kinase isoenzyme family, facilitates the transfer of high-energy phosphate from mitochondria to the cytosolic carrier, creatine. It exists as two isoenzymes: sarcomeric MtCK and ubiquitous MtCK, each encoded by separate genes. Unlike the exclusively dimeric cytosolic creatine kinase isoenzymes, mitochondrial creatine kinase forms dimers and octamers. Overexpression of ubiquitous mitochondrial creatine kinase, linked to elevated energy turnover and resistance to apoptosis, is observed in various malignant cancers with unfavorable prognoses.
Physical Appearance
A sterile, colorless solution.
Formulation
The solution contains 1mg/ml of CKMT1A antibody in a buffer of PBS at pH 7.4, supplemented with 10% glycerol and 0.02% sodium azide.
Storage Procedures
For short-term storage (up to 1 month), store the antibody at 4°C. For long-term storage, store at -20°C. Avoid repeated freeze-thaw cycles.
Stability / Shelf Life
The antibody is stable for 12 months when stored at -20°C and for 1 month at 4°C.
Applications
This antibody has been validated for ELISA and Western blot analysis to ensure specificity and reactivity. However, optimal working dilutions should be determined empirically for each application. A starting dilution of 1:1000 is recommended.
Synonyms
Creatine kinase mitochondrial 1A, creatine kinase mitochondrial 1 (ubiquitous), creatine kinase U-type mitochondrial, Acidic-type mitochondrial creatine kinase, Ubiquitous mitochondrial creatine kinase, CKMT1, U-MtCK, mia-CK, EC 2.7.3, EC 2.7.3.2.
Purification Method
CKMT1A antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
PAT17A2AT.
Immunogen
Anti-human CKMT1A mAb, clone PAT17A2AT, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human CKMT1A protein 40-417 amino acids purified from E. coli.
Ig Subclass
Mouse IgG2b heavy chain and k light chain.

Product Science Overview

Introduction

Creatine Kinase, Mitochondrial 1A (CKMT1A) is a protein-coding gene that plays a crucial role in cellular energy metabolism. It is part of the creatine kinase isoenzyme family, which is responsible for the transfer of high-energy phosphate from mitochondria to the cytosolic carrier, creatine .

Gene and Protein Structure

CKMT1A is encoded by the CKMT1A gene, which is located on chromosome 15. The protein exists in two isoenzymes: sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes . The mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes .

Function

CKMT1A plays a central role in energy transduction in tissues with large, fluctuating energy demands such as the brain. It catalyzes the reversible transfer of phosphate between ATP and various phosphagens (e.g., creatine phosphate) . This process is essential for maintaining cellular energy homeostasis, especially in tissues with high energy demands .

Expression and Localization

CKMT1A is ubiquitously expressed in mammals and is mainly localized to the mitochondrial intermembrane space . It is highly expressed in tissues such as the brain, appendix, tonsil, skin, and colon . The protein’s distribution within the cell includes both the mitochondrion and the cytosol .

Clinical Significance

Impairment in CKMT1A due to oxidative and radical-induced damage has been reported in numerous diseases, including neurodegenerative disorders . Overexpression of CKMT1A is observed in several tumors where high energy consumption is required for proliferation . Diseases associated with CKMT1A include Mitochondrial Complex I Deficiency, Nuclear Type 19, and Reticular Dysgenesis .

Mouse Anti Human CKMT1A Antibody

The mouse anti-human CKMT1A antibody is a monoclonal antibody used in various research applications, including Western blotting, immunohistochemistry, and immunocytochemistry . It is used to detect CKMT1A protein expression in human, mouse, and rat tissues . The antibody is purified by affinity chromatography and is available in different concentrations for research purposes .

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