anti-Homo sapiens (Human) CKMT2 Monoclonal Antibody raised in Mouse

Description

Applications in Research

CKMT2 monoclonal antibodies are widely used in molecular and cellular biology. Validated applications and protocols include:

Application	Dilution Range	Sample Types	Key Findings
Western Blot (WB)	1:500–1:8000	Tissue lysates (heart, skeletal muscle )	Detects CKMT2 in human HeLa cells , mouse heart , and xenograft models .
Immunohistochemistry (IHC)	1:500–1:2000	Paraffin-embedded tissues (e.g., colon cancer)	Overexpression observed in COAD and CHOL tumors .
Immunofluorescence (IF)	1:500	Cultured cells (e.g., HeLa)	Localizes CKMT2 to mitochondria .
ELISA	Variable	Plasma, serum	Quantifies CKMT2 in reperfusion injury studies .

Cancer Biology

Prognostic Biomarker: Low CKMT2 expression correlates with better immunotherapy outcomes in five cancer types (e.g., lung adenocarcinoma) .
Tumor Microenvironment: CKMT2 interacts with immune-related genes (e.g., TLRs, TRAF6) and regulates oxidative stress pathways .

Cardiovascular Research

Reperfusion Injury: Plasma CKMT2 levels rise post-reperfused myocardial infarction (MI), linking it to mitochondrial damage and impaired cardiac function .

Mitochondrial Dynamics

CKMT2 stabilizes mitochondrial membrane potential ( $\Delta \psi_m$ ) and modulates ROS production, critical for immune cell function .

Validation and Quality Control

CKMT2 monoclonal antibodies are rigorously validated:

Parameter	Data
Purity	>95% by SDS-PAGE
Specificity	Confirmed via knockout/knockdown models in published studies
Cross-Reactivity	No reactivity with non-target species under standard conditions
Storage	Stable at -20°C in PBS with 0.02% sodium azide

Future Directions and Limitations

Therapeutic Potential: CKMT2 inhibition may enhance immunotherapy efficacy in cancers with low CKMT2 expression .
Biomarker Utility: Plasma CKMT2 could aid in diagnosing reperfusion injury post-MI .
Limitations: Limited data on CKMT2 isoform-specific roles and off-target effects in polyclonal preparations .

Product Specs

Form

Purified mouse monoclonal antibody in a buffer solution composed of 0.1M Tris-Glycine (pH 7.4), 150 mM NaCl, 0.2% sodium azide, and 50% glycerol.

Lead Time

We typically ship orders within 1-3 business days of receipt. Delivery times may vary based on shipping method and destination. Please contact your local distributor for specific delivery time estimates.

Synonyms

SMTCK

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CKMT2 Monoclonal Antibody