CLDN11 Antibody, FITC conjugated
Description
Research Applications
The antibody is used to investigate CLDN11’s functions in:
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Spermatogenesis: CLDN11 forms tight junctions in Sertoli cells, preventing autoimmune responses by blocking IgG penetration into seminiferous tubules .
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Bone Metabolism: CLDN11 regulates osteoblast differentiation and inhibits osteoclastogenesis via Notch signaling .
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Neurological Studies: CLDN11 is a marker for oligodendrocytes in myelin sheath formation .
Product Comparisons
Immunofluorescence Protocol
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Fixation: 4% PFA (10 min).
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Permeabilization: 0.1% Triton X-100 (10 min).
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Blocking: 5% BSA (1 hr).
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Primary Antibody: 1:100–1:500 dilution (4°C overnight).
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Secondary Antibody: FITC-conjugated anti-rabbit IgG (1:500, 1 hr RT).
Western Blotting
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Sample: 50 μg lysate/lane.
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Membrane: PVDF, 0.45 μm.
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Blocking: 5% milk (1 hr).
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Primary Antibody: 1:1,000 (4°C overnight).
Research Findings
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Immunological Barriers: CLDN11 prevents IgG penetration into seminiferous tubules, reducing autoantibody production .
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Bone Homeostasis: CLDN11 enhances osteoblast differentiation via Smad1/5/8 phosphorylation and inhibits osteoclastogenesis by downregulating ERK signaling .
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Cancer Studies: Elevated CLDN11 expression is linked to epithelial-to-mesenchymal transition and metastasis in liver cancer .
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- Studies have shown that the expression of claudin-11 in cutaneous squamous cell carcinoma (cSCC) cells is dependent on the activity of p38delta MAPK. Knockdown of claudin-11 has been found to enhance cSCC cell invasion. PMID: 27992079
- Research has indicated that CLDN11 promoter hypermethylation is a frequent occurrence in LSCC and contributes to metastasis and progression of LSCC. PMID: 28743857
- The expression of claudin-11 and -23 has been observed to be significantly downregulated in gastric cancer. PMID: 28350854
- Claudin-11 may be a vital component of the blood-testis barrier in humans. PMID: 27486954
- An inverse correlation has been identified between the expression of miR-99b and CLDN11 levels. These findings suggest that elevated miR-99b expression is an independent prognostic factor associated with poor survival in patients with Hepatocellular carcinoma (HCC). PMID: 26134929
- CLDN11 has been identified as an epigenetic biomarker for malignancy in dysplastic nevus and melanoma. PMID: 24999589
- Research suggests that cancer cells may induce CLDN11 overexpression, leading to collective migration of peritumoral CAFs through TGF-beta secretion. PMID: 24268521
- Up-regulation of claudin-11 expression has been observed in gastric cancer tissue. PMID: 23919729
- The spatial organization of claudin-11 and connexin-43 has been found to be altered in men experiencing primary seminiferous tubule failure. PMID: 23706332
- Disorganization of claudin-11 expression in Sertoli cells may be a contributing factor to impaired spermatogenesis. PMID: 22951003
- Treatment with 3-deazaneplanocin A, an inhibitor of H3K27 methyltransferase, has been shown to attenuate CLDN11 induction by serum stimulation while concurrently maintaining sustained miR-1275 expression. PMID: 22736761
- Claudin-11 may play a role in preventing cancer progression and could serve as a therapeutic target for reducing metastasis. PMID: 21468549
- The late spermatogenic wave may negatively regulate claudin-11 gene activation and the subcellular localization of claudin-11 in Sertoli cells, thereby altering the blood testis barrier in the human testis. PMID: 20850723
- Expression of claudins 11 has been observed in meningiomas. PMID: 20546350
- Hypermethylation of CLDN11, leading to downregulated expression, contributes to gastric carcinogenesis by increasing cellular motility and invasiveness. PMID: 19956721
- The contribution of the tight junction protein CLDN11 to barrier function in endothelial cells is a novel finding and may reflect the hemodynamic requirements of the corpus cavernosum. PMID: 19622796
- Data indicates that in rhesus monkeys, immune responses directed at human OSP are encephalitogenic, leading to inflammatory responses throughout the central nervous system and selective demyelination of the optic nerve. PMID: 18412169
- The disruption of the blood-testis barrier is related to a dysfunction of claudin-11, rather than a failure of its expression. PMID: 19241088
