CLE46 Antibody

CLE Peptide Family Overview

CLE peptides are small signaling molecules critical for regulating plant development, including stem cell maintenance and vascular differentiation . While CLE46 is not explicitly discussed in available literature, CLE41/CLE44 (also known as TDIF, Tracheary Element Differentiation Inhibitory Factor) are well-characterized members of this family. These peptides bind to receptors like TDR (TDIF RECEPTOR) to modulate vascular stem cell fate .

Antibody Development for CLE Peptides

Antibodies targeting CLE peptides are pivotal for studying their localization, receptor interactions, and signaling mechanisms. Key examples include:

Anti-TDIF/CLE41/CLE44 Antibody

• Target: Synthetic TDIF peptide (HEVHypSGHypNPISN) .

• Application: Immunohistochemical staining in Arabidopsis seedlings to map peptide distribution .

• Function: Inhibits tracheary element differentiation in Zinnia cell cultures, demonstrating dose-dependent bioactivity .

Functional Insights from CLE Antibody Studies

• Vascular Development: CLE41 antibodies block tracheary element differentiation by competing with endogenous TDIF for TDR binding, confirming ligand-receptor specificity .

• Cross-Species Relevance: Synthetic hybrid peptides (e.g., KIN) combining CLE41 and CLV3 sequences exhibit dual bioactivity, highlighting structural conservation in CLE signaling .

Methodological Advances

• Photoaffinity Labeling: Radioiodinated ASA-TDIF and anti-HaloTag antibodies enable precise mapping of peptide-receptor interactions in plant membranes .

• Immunoprecipitation: Anti-TDIF antibodies coupled with protein A–Sepharose isolate receptor complexes for downstream analysis .

Comparative Analysis of CLE Antibody Applications

Future Directions

• Antibody Engineering: Develop CLE46-specific antibodies using synthetic peptide immunogens modeled after conserved CLE motifs .

• High-Throughput Screening: Adapt CLE41 antibody validation protocols (e.g., photoaffinity labeling) to explore CLE46 bioactivity .