Cleaved-GGT7 (T472) Antibody
Description
Definition and Target Specificity
The Cleaved-GGT7 (T472) Antibody is a polyclonal rabbit-derived immunoglobulin G (IgG) antibody designed to detect the activated form of the gamma-glutamyltransferase 7 (GGT7) enzyme. It specifically recognizes a cleaved epitope adjacent to threonine residue 472 (T472), which is exposed following proteolytic cleavage of the enzyme. This cleavage event is critical for GGT7 activation, enabling its role in glutathione metabolism and amino acid transpeptidation .
Biological Roles:
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Glutathione Metabolism: Facilitates the breakdown and recycling of glutathione, a key antioxidant.
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Transpeptidation: Mediates amino acid exchange, influencing cellular stress responses.
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Cancer Pathways: Interacts with CT120, a plasma membrane protein implicated in lung carcinogenesis .
Antibody Mechanism and Applications
The antibody detects endogenous GGT7 fragments generated by cleavage near T472, a hallmark of enzyme activation. This specificity allows researchers to study GGT7 activity in disease contexts, particularly oxidative stress and cancer .
Validated Applications:
| Application | Dilution Range | Notes |
|---|---|---|
| Western Blot (WB) | 1:500–1:2000 | Detects cleaved GGT7 in lysates . |
| ELISA | 1:10,000 | Quantitative analysis of GGT7 activation . |
Potential Research Directions:
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Oncology: Investigating GGT7 activation in tumor microenvironments linked to glutathione metabolism.
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Neurodegeneration: Exploring GGT7’s role in oxidative stress pathways (e.g., Alzheimer’s disease ).
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Toxicology: Monitoring enzyme activation in response to xenobiotics .
Product Characteristics
| Attribute | Value |
|---|---|
| Host | Rabbit |
| Isotype | IgG |
| Conjugate | Non-conjugated |
| Storage | -20°C or -80°C (avoid freeze-thaw cycles) |
Limitations:
Product Specs
Target Background
- Research indicates that GGT7 plays a crucial role in glioblastoma multiforme (GBM) growth. GGT7's involvement in tumorigenesis is likely mediated through its regulation of anti-oxidative damage. PMID: 25884624
- GGTL3 protein, a close relative of GGT7, interacts with CT120, a plasma membrane-associated protein potentially involved in lung carcinogenesis. PMID: 12270127
