Cleaved-ITIH1 (D672) Antibody
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Description
Target Overview
ITIH1 (Inter-alpha-trypsin inhibitor heavy chain H1) is a 911-residue secreted glycoprotein (101.4 kDa) involved in hyaluronan metabolism and extracellular matrix stabilization . The cleaved form (D672) represents a proteolytically activated fragment generated during specific biological processes, detectable exclusively by antibodies targeting this epitope .
Research Use Cases
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Proteolytic Activity Studies: Identifies activated ITIH1 fragments in inflammation or tissue remodeling contexts .
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Biomarker Validation: Quantifies cleaved ITIH1 levels in serum/plasma via ELISA for disease association studies .
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Pathway Analysis: Localizes ITIH1 activation sites in tissue sections using IHC .
Recommended Protocols
| Technique | Conditions |
|---|---|
| Western Blot | SDS-PAGE with 10% gel; transfer to PVDF membrane; block with 5% non-fat milk |
| ELISA | Coat plates with 2–4 μg/mL antigen; detect with HRP-conjugated secondary |
Cross-Reactivity Profile
Sensitivity Threshold
Handling Guidelines
Product Specs
Buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Form
Liquid
Lead Time
Typically, we can ship products within 1-3 business days after receiving your order. Delivery times may vary depending on the purchasing method or location. Please contact your local distributor for specific delivery details.
Synonyms
H1P antibody; IATIH antibody; IGHEP1 antibody; Inter alpha (globulin) inhibitor H1 antibody; Inter alpha (globulin) inhibitor H1 polypeptide antibody; Inter alpha trypsin inhibitor heavy chain 1 antibody; Inter alpha trypsin inhibitor heavy chain H1 antibody; Inter-alpha-inhibitor heavy chain 1 antibody; Inter-alpha-trypsin inhibitor complex component III antibody; Inter-alpha-trypsin inhibitor heavy chain H1 antibody; ITI heavy chain H1 antibody; ITI-HC1 antibody; ITIH antibody; ITIH1 antibody; ITIH1_HUMAN antibody; Serum-derived hyaluronan-associated protein antibody; SHAP antibody
Target Names
ITIH1
Uniprot No.
Target Background
Function
Cleaved-ITIH1 (D672) Antibody may serve as a carrier of hyaluronan in serum or as a binding protein between hyaluronan and other matrix proteins, including those on cell surfaces in tissues. This regulation of hyaluronan localization, synthesis, and degradation is essential for cells undergoing biological processes. The antibody also contains a potential peptide that could stimulate a broad spectrum of phagocytic cells.
Gene References Into Functions
- Thrombin treatment of the inflammatory matrix results in the dissolution of HC-HA cable structures and eliminates leukocyte adhesion. These findings establish a novel mechanism whereby thrombin cleavage of HC1 regulates the adhesive properties of an inflammatory HA matrix. PMID: 27679489
- Data suggest that Mg2+ or Mn2+ (but not Ca2+) induce a conformational change in inter-alpha-inhibitor (ITIH1 and ITIH2) and a bikunin/chondroitin sulfate-dependent increase in thermodynamic stability. Bikunin binds adjacent to the two heavy chains. PMID: 26728454
- The results of this study indicate that shared genetic risk factors for both schizophrenia and major depressive disorder are present in ITIH family genes within the Han Chinese population. PMID: 24389398
- Data indicate that TSG-6-mediated cross-linking of hyaluronan (HA) films is impaired in the presence of inter-alpha-inhibitor (IalphaI). This effect suppresses the TSG-6-mediated enhancement of HA binding to CD44-positive cells. PMID: 24005673
- Human inter-alpha-inhibitor is a substrate for factor XIIIa and tissue transglutaminase. PMID: 21939789
- Transfection into the H460M Human large cell lung carcinoma line decreased tumor metastasis in nude mice. PMID: 11807786
- PTX3 directly interacts with inter-alpha-trypsin inhibitor and plays a role in hyaluronan organization and cumulus oophorus expansion. PMID: 17675295
- The first systematic analysis of the differential expression of ITIH genes in human cancer, showing frequent downregulation, which may be associated with the initiation and/or progression of these malignancies. PMID: 18226209
Database Links
Protein Families
ITIH family
Subcellular Location
Secreted.
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