CLH2 Antibody
Description
Introduction
CLH2 is a mouse-derived monoclonal antibody specifically targeting MUC5AC, a high-molecular-weight glycoprotein critical in mucosal protection and implicated in cancer diagnostics. This antibody is widely utilized in research and clinical pathology for detecting MUC5AC expression patterns in normal and diseased tissues .
Applications in Research & Diagnostics
Primary Uses
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Immunohistochemistry (IHC): Detects MUC5AC in paraffin-embedded tissues (e.g., gastric carcinoma, biliary tract cancers) .
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Immunofluorescence (IF)/Flow Cytometry: Identifies MUC5AC in cell lines and fresh tissues .
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Western Blot (WB): Validated in studies analyzing mucin expression .
3.2 Diagnostic Utility
CLH2 aids in distinguishing:
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Primary ovarian mucinous tumors (MUC5AC+) vs. colorectal adenocarcinomas (MUC5AC-) .
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Pancreatic ductal carcinomas (MUC5AC+) vs. normal pancreas (MUC5AC-) .
Research Findings
Expression in Biliary Tract Cancers
| Tissue Type | CLH2 Positivity | Study |
|---|---|---|
| Intrahepatic cholangiocarcinoma | 93% (high-grade) | Okumura 2020 |
| Gallbladder dysplasia | 53% | Chang 2004 |
| Bile duct adenomas | 90% | Hughes 2010 |
Key Observations
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CLH2 detects immature MUC5AC in pre-cancerous lesions (e.g., biliary intraepithelial neoplasia) .
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Overexpression correlates with poor prognosis in pancreatic and gastric cancers .
Validation & Quality Control
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Specificity: Confirmed using CRISPR-generated MUC5AC knockout cell lines .
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Reproducibility: Cited in 7+ peer-reviewed studies, including PMC articles .
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Limitations: Does not bind heavily glycosylated (mature) MUC5AC, necessitating complementary antibodies for full mucin analysis .
Clinical Relevance
CLH2’s role in identifying MUC5AC overexpression has implications for:
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- Studies have shown that the mRNA levels of two chlorophyllase genes in Arabidopsis, AtCLH1 and AtCLH2, significantly increase upon exposure to white light. This suggests a role for these enzymes in chlorophyll catabolism. PMID: 21896889
- Further research proposes that AtCLH1 plays a role in plant damage control and can regulate the balance between different plant defense pathways. PMID: 15598807
- While AtCLHs are involved in chlorophyll breakdown, they are not essential for senescence-related chlorophyll degradation in vivo. This suggests that the true chlorophyllase involved in this process remains to be identified. PMID: 17996203
- Research indicates that AtCLH2 may be required at the initial stage of leaf senescence, potentially playing a role in converting chlorophyll b to chlorophyll a. PMID: 18349515
