CLSTN3 Antibody, FITC conjugated

Introduction

The CLSTN3 Antibody, FITC conjugated is a fluorescently labeled antibody designed for the immunodetection of Calsyntenin-3 (CLSTN3), a transmembrane adhesion protein involved in synaptic and metabolic processes. This article provides a comprehensive overview of the antibody’s specifications, applications, and research relevance, supported by diverse scientific sources.

Western Blotting (WB)

• The antibody is validated for detecting CLSTN3 in Western blotting, with protocols emphasizing the use of 1x PBS buffer and sodium azide as a preservative .

• Dilution Recommendation: 1:500–1:1000 in PBS with 10% FBS .

Immunofluorescence (IF)

• FITC conjugation enables visualization of CLSTN3 localization in fixed cells or tissues. A recommended dilution of 1:200–1:500 is suggested for mammalian cell IF .

Synaptic Function

• CLSTN3 interacts with neurexins (NRXNs) to induce presynaptic differentiation . Studies using CLSTN3 antibodies (including FITC-conjugated variants) have shown its role in clustering synaptic proteins like synapsin and bassoon .

Metabolic Regulation

• Overexpression of CLSTN3 in adipose tissue correlates with improved glucose tolerance and reduced hepatic steatosis . FITC-conjugated antibodies have been used to study CLSTN3 localization in ER–lipid droplet contact sites .

Cancer and Disease Models

• CLSTN3 antibodies are critical in studying its involvement in neurodegenerative diseases (e.g., Alzheimer’s) and obesity-related pathologies .

Comparative Analysis of CLSTN3 Antibodies