CBL6 Antibody
Description
CBL6 Antibody in Drosophila Research
The Dcbl Ex6 8C4 antibody, developed by the Developmental Studies Hybridoma Bank (DSHB), targets the Drosophila D-cbl protein, a member of the Cbl family involved in signal transduction and protein ubiquitination.
Key Features:
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Host Species: Mouse (IgG1 isotype)
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Applications: Immunofluorescence (IF), Western Blot (WB), Immunocytochemistry (ICC)
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Immunogen: Recombinant 6X-His D-Cbl fusion protein
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Reactivity: Specific to Drosophila melanogaster
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Molecular Weight: 51.4 kDa
Research Applications:
This antibody has been instrumental in studying the role of D-cbl in Drosophila development, particularly in cell signaling pathways and ubiquitin-mediated protein degradation .
CBL6 Antibody in Plant Biology
Mouse-derived antibodies targeting Calcineurin B-like protein 6 (CBL6) in plants, such as Oryza sativa (rice) and Arabidopsis thaliana, are used to investigate calcium signaling and stress responses.
Key Antibody Variants:
| Product ID | Host Species | Reactivity | Applications | Conjugates Available |
|---|---|---|---|---|
| CBMOAB-22055FYB | Mouse | Rice, A. thaliana | WB, ELISA | AP, APC, Biotin, FITC, HRP |
| MO-AB-27303W | Mouse | Cottonwood, A. thaliana | WB, ELISA | Cy3, Cy5, PE, PerCP |
Functional Insights:
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Role of CBL6: Regulates calcium-dependent signaling pathways under abiotic stress (e.g., salinity, drought).
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Experimental Use: These antibodies enable protein localization studies and quantification via Western blotting and ELISA .
Comparative Analysis of CBL6 Antibodies
| Parameter | Dcbl Ex6 8C4 (DSHB) | CBMOAB-22055FYB (Creative Biolabs) |
|---|---|---|
| Target Species | Drosophila melanogaster | Oryza sativa, A. thaliana |
| Primary Use | Developmental biology | Plant stress response mechanisms |
| Conjugate Options | None specified | Multiple (AP, APC, Biotin, etc.) |
| Binding Specificity | D-cbl protein | Calcineurin B-like protein 6 |
Research Limitations and Future Directions
While existing CBL6 antibodies are critical for basic research, their utility is confined to model organisms (e.g., Drosophila and plants). No clinical or therapeutic applications have been reported for CBL6-targeting antibodies. Future studies could explore:
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Cross-reactivity with mammalian CBL homologs.
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Structural characterization of antibody-epitope interactions.
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- Research has shown that CBL6 targeting is facilitated by lipid modification through S-acylation. An N-terminal peptide of 22 amino acids is both necessary and sufficient for directing tonoplast targeting. PMID: 29023681
