CAPH2 Antibody
Description
Biological Function of CAPH2
CAPH2 forms part of the condensin II complex, which ensures mitotic chromosome architecture and prevents DNA damage. Key functions include:
-
Chromatin compaction during prophase
-
Resolution of sister chromatids
-
Telomere stability maintenance
Mutations in CAPH2 (e.g., R551P, R551S, S556F) disrupt condensin II function, leading to:
-
Increased chromosomal instability (CIN)
-
Telomere-associated DNA damage
Table 1: CAPH2 Antibody Comparison
Cross-Reactivity Challenges
The Bethyl A302-276A antibody exhibits significant off-target effects:
-
Co-precipitates SWI/SNF components (SMARCA4, SMARCC1) in immunoprecipitation-mass spectrometry (IP-MS)
-
Fails to distinguish NCAPH2 depletion in auxin-inducible degradation systems
-
Custom antibodies show superior specificity for condensin II studies
Mutation-Specific Phenotypes
Cancer-associated CAPH2 mutations impair condensin II through:
Table 2: Functional Impacts of CAPH2 Mutations
| Mutation | Chromatin Binding | SMC4 Interaction | Cellular Consequences |
|---|---|---|---|
| R551P | ↓ 65% | ↓ 78% | Telomere bridges, ATR-dependent damage |
| R551S | ↓ 58% | ↓ 82% | Micronuclei formation, aneuploidy |
| S556F | ↓ 72% | ↓ 91% | Chromosomal breaks, mitotic catastrophe |
Experimental Validation Guidelines
For reliable CAPH2 detection:
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
CAPH2 is a regulatory subunit of the condensin-II complex. This complex plays a crucial role in organizing and stabilizing chromosomes, contributing significantly to mitotic chromosome architecture. Evidence suggests condensin-II is involved in DNA damage repair and genome protection against genotoxic stressors, such as boron excess, zeocin, and aphidicolin.
- HEB1 and HEB2 genes encode subunits of the condensin II complex. Studies have shown that mutants exhibit increased sensitivity to DNA double-strand breaks induced by excess boron. (PMID: 21917552)
- The localization of condensin subunits AtCAP-H and AtCAP-H2 demonstrates dynamic changes throughout the mitotic cell cycle. AtCAP-H2 is consistently localized to nuclear or chromosomal regions. (PMID: 15883832)
