CNGC11 Antibody
Description
CNGC11 Antibody: Core Characteristics
CNGC11 antibodies target the cyclic nucleotide-gated ion channel subunit alpha 11, a non-selective cation channel involved in calcium (Ca²⁺) transport and pathogen defense signaling . These antibodies are typically monoclonal or polyclonal reagents validated for techniques such as:
Key epitopes for CNGC11 antibodies often reside in cytoplasmic domains, such as the cyclic nucleotide-binding domain (CNBD) or C-terminal regulatory regions .
Role in Plant Immunity
CNGC11 antibodies have been instrumental in characterizing autoimmune phenotypes in Arabidopsis mutants. Studies show:
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Constitutive defense activation: Mutants like cpr22 (a CNGC11/12 chimera) exhibit spontaneous cell death and elevated salicylic acid levels, detectable via antibody-based protein analysis .
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Calcium signaling: CNGC11 mediates Ca²⁺ influx during pathogen responses, as confirmed by electrophysiological assays using antibodies for protein localization .
Key Studies Utilizing CNGC11 Antibodies
Technical Validation and Challenges
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Specificity: Anti-His tag antibodies are frequently used for recombinant CNGC11 detection, but custom antibodies targeting unique epitopes improve specificity .
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Cross-reactivity: Some CNGC antibodies (e.g., NeuroMab L36/12) detect multiple subunits (CNGA1/CNGA3), necessitating validation for CNGC11 exclusivity .
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Functional assays: Combining antibody-based detection with electrophysiology (e.g., patch-clamp) resolves channel activity dynamics .
Future Directions
Ongoing research aims to:
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
CNGC11 and CNGC12: Function and Interactions
- Intersubunit Interactions: Residues crucial for intersubunit interactions between CNGC12 and CNGC11 have been identified (PMID: 23735507).
- Role in Plant Immunity and Development: AtCNGC11 and AtCNGC12 mediate calcium signaling involved in plant immunity, gravitropic bending, and senescence (PMID: 22041991).
- Senescence Regulation: AtCNGC11 and AtCNGC12 gene expression is induced during dark-induced senescence. Knockout mutants exhibit enhanced chlorophyll loss, with a more pronounced effect in the double mutant, suggesting synergistic roles in senescence regulation (PMID: 21414958).
- Cyclic Nucleotide Binding Domain: The α-helix within the cyclic nucleotide binding domain is functionally significant for CNGC11 and CNGC12 (PMID: 20378667).
- Chimeric Gene Formation: A 3-kb deletion can fuse the ATCNGC11 and ATCNGC12 genes, creating a novel chimeric gene, ATCNGC11/12 (PMID: 16461580).
