CNKSR2 Antibody
Description
Development and Production
The CNKSR2 antibody (Catalog #A08861-1) is manufactured by Boster Bio and targets a peptide sequence spanning amino acids 641–690 of the human CNKSR2 protein . It is validated for use in ELISA, immunohistochemistry (IHC), and Western blotting (WB). Key specifications include:
| Parameter | Details |
|---|---|
| Host | Rabbit |
| Immunogen | Synthetic peptide (AA 641–690) |
| Reactivity | Human, Mouse, Rat |
| Storage | -20°C for long-term; 4°C for short-term |
| Recommended Dilutions | WB: 1:500–1:2000; IHC: 1:100–1:300; ELISA: 1:20,000 |
The antibody is supplied in a PBS-based buffer with 50% glycerol and 0.02% sodium azide to prevent degradation .
Neurodevelopmental Studies
CNKSR2 is implicated in synaptic assembly and neurodevelopmental disorders. The antibody has been used to study its role in hippocampal granule neuron development, where it interacts with CYTH2 to stabilize synaptic signaling complexes . For example, co-immunoprecipitation assays confirmed CNKSR2’s binding to CYTH2, which prevents its proteasomal degradation .
Cancer Research
In cervical squamous cell carcinoma (CESC), high CNKSR2 expression correlates with improved survival outcomes and immune infiltration . Immunohistochemical analysis using this antibody revealed CNKSR2 localization in normal adjacent tissues vs. tumor samples, supporting its role as a prognostic biomarker .
Western Blot Validation
Boster Bio’s validation data demonstrate the antibody’s specificity in detecting CNKSR2 in 293T cell lysates (Figure 1, WB analysis) . Cross-reactivity with unrelated proteins (e.g., GFP) was not observed, ensuring high specificity .
Survival Analysis
Kaplan-Meier curves from TCGA cohorts show that high CNKSR2 expression (detected via this antibody) is associated with:
Immune Infiltration
Single-sample GSEA revealed CNKSR2’s correlation with NK and T-cell infiltration in CESC, suggesting its role in modulating tumor microenvironments .
Challenges and Considerations
-
Cross-reactivity: While the antibody shows specificity for CNKSR2, researchers should confirm results using orthogonal methods (e.g., RNAi knockdown).
-
Tissue Variability: CNKSR2 expression levels vary across cancers (e.g., downregulated in ovarian and pancreatic cancers) , necessitating tissue-specific optimization.
Product Specs
Target Background
- Elevated CNKSR2 expression has been correlated with breast cancer progression. PMID: 29534682
- Deletions in the CNKSR2 gene have been identified as a novel cause of X-linked intellectual disability and seizures. PMID: 25754917
- Research indicates that CNKSR2 deficiency plays a role in neurological systems associated with common neurological conditions. PMID: 25223753
- Through its interaction with Raf and Rlf, CNK2 may act as a regulator of Ras signaling. PMID: 14597674
- CNK and HYP, through their SAM domains, form a distinct dimer to mediate RAF kinase signaling. PMID: 18287031
