CNNM3 Antibody, HRP conjugated

Overview of CNNM3 and Its Antibody

CNNM3 (Cyclin M3) is a 707-amino acid transmembrane protein involved in magnesium ion homeostasis and metal transport . Despite its name, it lacks cyclin-like functions and contains two cystathionine β-synthase (CBS) domains critical for ligand binding . The HRP-conjugated antibody targets specific epitopes of CNNM3, facilitating its detection in experimental workflows.

Buffer Composition and Compatibility

The antibody’s performance depends on buffer conditions. Critical components include:

Recommended Dilutions

Role in Magnesium Homeostasis

CNNM3 interacts with TRPM7, a ion channel-kinase, to regulate cellular magnesium levels. Studies show CNNM3 co-expression reduces TRPM7 kinase activity, as evidenced by decreased phosphorylation at Ser1511 .

Cancer and Metastasis

CNNM3 binds phosphatase PRL-3, a driver of metastasis. Nanobodies disrupting this interaction inhibit tumor growth in preclinical models .

Subcellular Localization

CNNM3 localizes to the plasma membrane and cytoplasm, as confirmed by immunofluorescence in A431 cells .

Performance and Stability

HRP conjugates degrade over time, especially when diluted or exposed to heat. Stabilizers like LifeXtend™ enhance longevity .

Technical Considerations

• Cross-Reactivity: Predicted reactivity with dog samples .

• Molecular Weight: Observed at ~85 kDa (vs. predicted 76 kDa) due to glycosylation .

• Controls: Use HEK293 cells transfected with FLAG-tagged CNNM3 for WB validation .