Customize Os02g0134000 Antibody

Product Specs

Buffer

Preservative: 0.03% ProClin 300. Constituents: 50% Glycerol, 0.01M PBS, pH 7.4.

Form

Liquid

Lead Time

14-16 week lead time (made-to-order)

Synonyms

Os02g0134000 antibody; LOC_Os02g04120 antibody; OsJ_05277 antibody; P0030G11Phosphopantetheine adenylyltransferase 2 antibody; EC 2.7.7.3 antibody; Dephospho-CoA pyrophosphorylase 2 antibody; Pantetheine-phosphate adenylyltransferase 2 antibody

Target Names

Os02g0134000

Uniprot No.

B9F2L1

Target Background

Function

This antibody targets a protein with the following enzymatic activity: reversible adenylyl group transfer from ATP to 4'-phosphopantetheine, producing dephospho-CoA (dPCoA) and pyrophosphate. The enzyme does not utilize 4'-phosphopantothenoylcysteine as a substrate.

Protein Families

Eukaryotic CoaD family

Q&A

Here’s a structured, research-oriented FAQ for Os02g0134000 antibody applications, synthesized from peer-reviewed methodologies and technical guidelines:

Advanced Research Questions

How to resolve contradictory localization data between studies?

Root-cause analysis:
- Compare fixation methods (e.g., formaldehyde vs. methanol) .
- Validate with orthogonal methods (e.g., in situ hybridization vs. antibody staining) .
- Check batch-to-blot variability using recombinant monoclonals .

Strategies for epitope mapping of Os02g0134000 antibodies

Method: Phage display or peptide array using 15-mer overlapping fragments.
Analysis: Identify consensus motifs via MEME Suite; validate with alanine-scanning mutants .

Can this antibody distinguish phosphorylation states?

Experimental design:
- Treat samples with λ-phosphatase.
- Perform 2D gel electrophoresis + Western blot.
- Use Phos-tag™ gels for mobility shifts .
Limitation: Requires independent MS validation of modification sites .

Methodological Best Practices

Quantitative analysis of Os02g0134000 expression

Step	Protocol	Quality Control
Normalization	Spike-in recombinant protein (10 pg–1 ng)	R² >0.98 in standard curve
Signal detection	ChemiDoc™ with linear dynamic range	Avoid saturated pixels
Statistical analysis	Mixed-effects model for biological replicates	FDR <5%

Multiplexing with Os02g0134000 antibody

Panel design:
- Primary host: Rabbit (avoid cross-species interference)
- Secondary conjugates: Alexa Fluor 488 (Ex/Em 495/519) + IRDye 800CW
Validation: Spectral unmixing controls using single-antibody stains .

Troubleshooting Guide

No signal in Western blot

Cause	Solution	Validation
Over-fixation	Reduce formaldehyde exposure to 10 min	Compare fresh vs. fixed samples
Epitope masking	Antigen retrieval (10 mM citrate, 95°C, 20 min)	IHC-positive control tissue
Antibody degradation	Aliquot in stabilizing buffer (0.1% BSA, 0.02% NaN₂)	ELISA activity assay

High background in immunofluorescence

Optimization:
- Increase blocking time (overnight at 4°C) .
- Use Fab fragment secondary antibodies .
- Titrate Triton X-100 (0.01–0.3%) to balance permeabilization and protein retention .

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Os02g0134000 Antibody