cogc-6 Antibody

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Description

Target Protein: COG Complex Subunit 6

Protein Name:

  • Conserved oligomeric Golgi complex subunit 6 (COGC-6)

  • Component of oligomeric Golgi complex 6

Gene Name:

  • cogc-6

Cross-References:

Key Uses:

  • Western Blot (WB): Detects COGC-6 at ≈1 ng sensitivity .

  • Immunofluorescence (IF): Validated for Golgi complex localization studies.

  • ELISA: High-affinity binding confirmed via antibody-antigen interaction assays .

Technical Notes:

  • Antibody combinations are pre-mixed for immediate use.

  • Epitope determination services ($100 per combination) enable deconvolution of individual mAbs post-purchase .

Standard Packages (AbInsure™ Coverage):

End-UsePackageIncluded AntibodiesPriceAvailability
WBX1-Q21270X-Q21270 -N$59930 days
IFX2-Q21270X-Q21270 -N, X-Q21270 -C$89930 days

À La Carte Options (No AbInsure™ Coverage):

AntibodyPriceAvailability
X-Q21270 -N$59930 days
X-Q21270 -C$59930 days
X-Q21270 -M$59930 days

Custom Development:

  • Tailored mAbs for specific needs (e.g., epitope specificity, cross-reactivity) start at $599 with a 5–30 day turnaround .

Mechanistic Insights from Related Antibody Studies

While COGC-6 antibodies are primarily research tools, studies on other monoclonal antibodies (e.g., anti-CD6 mAbs like UMCD6) highlight broader therapeutic principles:

  • Cancer Immunotherapy: Anti-CD6 mAbs enhance NK cell cytotoxicity by upregulating NKG2D and perforin .

  • Autoimmune Disease: Anti-CD6 mAbs (e.g., itolizumab) suppress Th17 responses without inducing lymphopenia .

Product Specs

Buffer
Preservative: 0.03% Proclin 300
Composition: 50% Glycerol, 0.01M Phosphate Buffered Saline (PBS), pH 7.4
Form
Liquid
Lead Time
Made-to-order (14-16 weeks)
Synonyms
cogc-6 antibody; K07C11.9Conserved oligomeric Golgi complex subunit 6 antibody; COG complex subunit 6 antibody; Component of oligomeric Golgi complex 6 antibody
Target Names
cogc-6
Uniprot No.

Target Background

Function
Essential for normal Golgi function.
Database Links

KEGG: cel:CELE_K07C11.9

STRING: 6239.K07C11.9

UniGene: Cel.5253

Protein Families
COG6 family
Subcellular Location
Golgi apparatus membrane; Peripheral membrane protein.

Q&A

Here’s a structured collection of FAQs for researchers working with cogc-6 Antibody (Code: CSB-PA637418XA01CXY, Uniprot: Q21270), tailored to academic research scenarios and synthesized from interdisciplinary methodologies:

Advanced Research Questions

How to investigate cogc-6’s role in protein trafficking using co-localization studies?

  • Design:

    • Combine anti-cogc-6 with organelle-specific markers (e.g., Golgi, ER) in IHC.

    • Use super-resolution microscopy to resolve subcellular localization.

    • Controls: Include RNAi-treated C. elegans to confirm phenotype dependency .

  • Data analysis: Quantify Pearson’s correlation coefficient using ImageJ or Volocity.

How to resolve discrepancies in cogc-6 expression levels across developmental stages?

  • Approach:

    • Perform time-course WB/IHC on synchronized C. elegans populations (L1 to adult stages).

    • Normalize data to housekeeping proteins (e.g., actin) and account for batch effects using linear mixed models.

    • Contradiction analysis: If results conflict with literature, validate via qPCR or CRISPR-Cas9 tagging .

Methodological Comparison Table

ApplicationOptimal TechniqueKey ParametersValidation Criterion
Protein LocalizationIHC + Confocal MicroscopyFixation: 4% PFA
Primary antibody dilution: 1:200
Co-staining with organelle markers
Quantitative AnalysisWB + DensitometryLoading control: α-tubulin
Exposure time: 1–5 min
Linear signal range confirmed via serial dilution
Functional StudiesRNAi Knockdown + Phenotypic AssaydsRNA delivery: Feeding method
Phenotype: Embryonic lethality
Rescue via transgenic overexpression

Troubleshooting Data Table

IssuePotential CauseSolution
High background in IHCNon-specific bindingIncrease blocking time (2 hrs) or use 1% BSA instead of milk
Multiple WB bandsProtein degradation or isoformsFresh protease inhibitors; validate via deglycosylation (e.g., PNGase F)
Inconsistent staining across replicatesAntibody lot variabilityPre-test new lots with positive/negative controls

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