COL11 Antibody

Antibody Applications in Research

COL11 antibodies are validated across diverse methodologies:

Key Applications

Role in Disease

• Genetic Disorders: Mutations in COL11A1 cause Stickler syndrome, Marshall syndrome, and fibrochondrogenesis, leading to skeletal and ocular abnormalities .

• Cancer: Overexpression correlates with poor prognosis, chemoresistance, and immune infiltration in lung adenocarcinoma, breast cancer, and ovarian cancer .

Mechanistic Insights

• ECM Remodeling: COL11A1 secreted by cancer-associated fibroblasts (CAFs) promotes tumor stiffness and metastasis .

• Developmental Biology: Critical for zebrafish craniofacial cartilage formation, validated via knockout models .

Validation Criteria

• Specificity: Confirmed via COL11A1 knockout zebrafish and peptide competition assays .

• Cross-Reactivity: Human-specific in most cases; zebrafish-specific antibodies available .

Technical Considerations

• Dilution Optimization: Titration required for IHC/IF due to tissue-specific antigen availability .

• Storage: Stable at -20°C to -80°C in glycerol-containing buffers .