COL14 Antibody

Target Overview

COL14A1 encodes the alpha-1 chain of collagen XIV, a 193.5 kDa protein comprising 1,796 amino acids . It plays adhesive roles in collagen bundle integration and regulates fibrillogenesis, mechanical stress responses, and cell differentiation . COL14 is predominantly expressed in connective tissues, including lung, skin, and blood vessels .

Antibody Characteristics

COL14 antibodies are available in polyclonal and monoclonal forms, with the following specifications:

Fibrillogenesis Regulation

COL14 antibodies revealed that collagen XIV knockout mice (Col14a1⁻/⁻) exhibit disorganized dermal collagen fibrils and compromised tissue integrity, underscoring its role in fibril assembly .

Pulmonary Fibrosis

In idiopathic pulmonary fibrosis (IPF), COL14 expression is proportionally reduced relative to collagen I (COL1). Single-cell RNA sequencing shows elevated COL14A1 in IPF stromal cells, yet its protein ratio to COL1 decreases, suggesting dysregulated ECM remodeling .

Developmental Biology

COL14 is essential for cardiac interstitial formation. Embryonic Col14a1⁻/⁻ mice show ventricular wall thinning and impaired fibroblast proliferation .

Validation and Quality Control

• Specificity: Validated via immunoblotting and collagenase digestion .

• Cross-Reactivity: Confirmed in human, mouse, rat, and pig tissues .

• Citations: Cited in studies investigating fibrosis , developmental defects , and fibrillogenesis .

Limitations and Restrictions

• Diagnostic Use: Restricted to research; not approved for clinical diagnostics .

• Batch Variability: Polyclonal antibodies may exhibit lot-to-lot variability .

Future Directions

Current research leverages COL14 antibodies to explore therapeutic targeting in fibrotic diseases and tissue engineering. Ongoing studies focus on COL14’s interaction with TGF-β signaling pathways in IPF .