COPS4 Antibody
Description
Overview of COPS4
COPS4 (Gene ID: 51138) is a subunit of the COP9 signalosome, which shares structural and functional similarities with the 19S regulatory particle of the proteasome . It plays a role in:
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Ubiquitin-proteasome regulation: Mediating deneddylation of cullin subunits in SCF-type E3 ligases, modulating their activity .
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Circadian rhythms: Participating in light-dependent degradation of clock proteins (e.g., Timeless in Drosophila) .
Key Applications
Validation Highlights
Role in Disease
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Cerebral Ischemia/Reperfusion Injury: COPS4 stabilizes suppressor of cytokine signaling 3 (SOCS3), mitigating neuroinflammation .
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Cancer: The COP9 complex, including COPS4, influences tumor progression by regulating ubiquitination pathways .
Mechanistic Insights
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Ubiquitination: COPS4 modulates SCF ligase activity, affecting substrate degradation .
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Circadian Regulation: Interacts with molecular clock components in Drosophila .
Protocols and Usage Notes
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Antigen Retrieval: For IHC, use TE buffer (pH 9.0) or citrate buffer (pH 6.0) .
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Storage: Stable for one year at -20°C; avoid repeated freeze-thaw cycles .
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Titration: Optimize concentrations for specific experimental conditions .
Future Directions
Research on COPS4 is expanding into:
Product Specs
Target Background
- COPS4 has been identified as a novel binding partner of torsin A, the subunit 4 (CSN4) of the CSN signalosome. PMID: 21102408
- The level of COPS4 in fetal Down syndrome brain has been found to be comparable to that of controls. PMID: 11824616
