COX15 Antibody

COX15 Protein Function and Clinical Relevance

COX15 (cytochrome c oxidase assembly homolog 15) is a mitochondrial membrane protein essential for converting heme O to heme A, a prosthetic group required for COX activity . Deficiencies in COX15 disrupt oxidative phosphorylation, leading to mitochondrial disorders such as:

• Leigh syndrome (subacute necrotizing encephalomyelopathy)

• Hypertrophic cardiomyopathy

• Oocyte ferroptosis and female infertility

Mutations in COX15 impair heme A synthesis, stalling COX assembly and reducing mitochondrial respiration .

Key Applications of COX15 Antibodies

COX15 antibodies are widely used in:

• Western blot (WB) to detect endogenous COX15 levels (~44–55 kDa)

• Immunohistochemistry (IHC) for tissue-specific localization (e.g., heart, brain)

• Functional studies of COX15 interactions with mitochondrial complexes (e.g., cytochrome bc1 and COX)

Mechanistic Studies

• Ferroptosis in Oocytes: COX15 deficiency disrupts Fe²⁺/ROS homeostasis, triggering ferroptosis and oocyte defects .

• Heme A Biosynthesis: Overexpression of COX15 rescues heme A levels and COX activity in patient fibroblasts .

• Protein Interactions: COX15 associates with cytochrome bc1 complexes and Shy1, influencing COX assembly .

Clinical Correlations

Suppliers and Validation

Leading suppliers include Thermo Fisher (PA5-103383, PA5-34681), Abcam (ab201082), Proteintech (11441-1-AP), and Assay Genie (CAB14665). Antibodies are validated for:

• Specificity: Epitope mapping and knockout validation

• Cross-reactivity: Human, mouse, rat tissues

• Performance: Consistent detection in high-oxidative tissues (e.g., heart, brain)