Customize cox-5A Antibody

Description

Definition and Biological Role of COX5A

COX5A (Cytochrome c oxidase subunit Va) is a mitochondrial protein essential for cellular respiration as part of the cytochrome c oxidase (COX) complex, the terminal enzyme of the electron transport chain . It facilitates proton gradient formation for ATP synthesis and regulates mitochondrial energy metabolism . COX5A antibodies are tools for detecting and studying this protein in human, mouse, rat, and other species via techniques like Western blot (WB), immunohistochemistry (IHC), immunoprecipitation (IP), and immunofluorescence (IF) .

Aging-Related Cognitive Decline

Mechanism: COX5A downregulation in aged hippocampus correlates with reduced BDNF/ERK1/2 signaling, impairing dendritic branching and spatial memory .
Intervention: Transgenic mice with COX5A overexpression (51% increase) showed improved hippocampal mitochondrial activity, ATP levels, and memory performance .
Key Finding: ERK1/2 inhibitor PD98059 blocked COX5A’s neuroprotective effects, confirming BDNF/ERK1/2 pathway dependency .

Doxorubicin-Induced Cardiotoxicity

Protective Role: COX5A overexpression in cardiomyocytes reduced oxidative stress (ROS), mitochondrial dysfunction, and apoptosis via PI3K/Akt signaling .
Clinical Relevance: Human end-stage dilated cardiomyopathy (DCM) samples showed diminished COX5A expression, suggesting therapeutic potential .

Systemic Lupus Erythematosus (SLE)

OxPhos Signature: COX5A is a hub gene in SLE T cells, correlating with disease activity, skin/kidney involvement, and interferon response .
Biomarker Potential: Elevated COX5A expression in effector T cells predicts organ damage severity .

COX5A Antibody Product Comparison

Product	Host/Isotype	Reactivity	Applications	Vendor
A-5 (SCBT)	Mouse IgG1 κ	Human, Mouse, Rat	WB, IP, IF, IHC, ELISA	Santa Cruz Biotech
11448-1-AP	Rabbit IgG	Human, Mouse, Rat	WB, IHC, IF, ELISA	Proteintech
EPR14208(B)	Rabbit Monoclonal	Human, Mouse, Rat	WB, IHC, IP	Abcam
NBP3-06072	Rabbit Polyclonal	Human	WB, IP, IHC-P	Novus/Bio-Techne
60452-1-PBS	Mouse Monoclonal	Human	Cytometric bead array	Proteintech

Critical Technical Considerations

Antigen Retrieval: IHC requires pH 6.0–9.0 buffer pretreatment for optimal signal .
Dilution Optimization: WB dilutions vary widely (1:500–1:5000), necessitating titration .
Conjugation Options: Some antibodies (e.g., A-5) are available in HRP, PE, FITC, or Alexa Fluor® forms .

Product Specs

Buffer

Preservative: 0.03% ProClin 300; Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Lead Time

14-16 weeks (Made-to-order)

Synonyms

cox-5A antibody; cco-2 antibody; Y37D8A.14Cytochrome c oxidase subunit 5A antibody; mitochondrial antibody; Cytochrome c oxidase polypeptide Va antibody

Target Names

cox-5A

Uniprot No.

P55954

Target Background

Function

COX-5A is a component of cytochrome c oxidase (Complex IV, CIV), the terminal enzyme in the mitochondrial electron transport chain (ETC). This enzyme is crucial for oxidative phosphorylation, the process by which cells generate ATP. The ETC comprises three multi-subunit complexes: succinate dehydrogenase (Complex II, CII), ubiquinol-cytochrome c oxidoreductase (Complex III, CIII), and cytochrome c oxidase (Complex IV, CIV). These complexes work cooperatively to transfer electrons from NADH and succinate to molecular oxygen, establishing an electrochemical gradient across the inner mitochondrial membrane. This gradient powers both transmembrane transport and ATP synthase. Cytochrome c oxidase specifically catalyzes the reduction of oxygen to water. Electrons from reduced cytochrome c in the intermembrane space are transferred through the dinuclear copper A center (Cu_A) of subunit II and heme A of subunit I to the active site in subunit I. This active site is a binuclear center (BNC) composed of heme a₃ and copper B (Cu_B). The BNC utilizes four electrons from cytochrome c and four protons from the mitochondrial matrix to reduce molecular oxygen to two water molecules.

Database Links

KEGG: cel:CELE_Y37D8A.14

STRING: 6239.Y37D8A.14.2

UniGene: Cel.8875

Protein Families

Cytochrome c oxidase subunit 5A family

Subcellular Location

Mitochondrion inner membrane; Peripheral membrane protein; Matrix side.

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cox-5A Antibody