CYP26-2 Antibody

Overview of CYP26-2 Antibody

The CYP26-2 antibody is designed for immunodetection of the CYP26-2 protein in biological samples. Unlike mammalian CYP26 enzymes, which regulate retinoid signaling, plant CYP26 enzymes are less studied. In Arabidopsis, CYP26-2 is hypothesized to participate in metabolic pathways involving plant-specific substrates, though its exact function remains unclear .

Antibody Validation

• The CYP26-2 antibody has been validated for Western blot and ELISA, with reactivity confirmed in Arabidopsis lysates .

• No peer-reviewed studies detailing its use in functional assays (e.g., enzyme activity inhibition) were identified in the provided sources.

Comparison with Mammalian CYP26 Enzymes

A comparison of key features between plant CYP26-2 and mammalian CYP26 enzymes highlights functional divergences:

Limitations and Knowledge Gaps

• Functional Data: No studies in the provided sources elucidate CYP26-2’s enzymatic activity or biological role in plants.

• Cross-Reactivity: Antibody specificity for CYP26-2 versus other plant P450s is unverified.

• Evolutionary Context: The relationship between plant CYP26-2 and mammalian CYP26 enzymes remains unexplored.

Future Directions

• Substrate Identification: Mass spectrometry or metabolomics could identify plant metabolites processed by CYP26-2.

• Genetic Studies: CRISPR knockout lines in Arabidopsis may reveal phenotypic impacts of CYP26-2 loss.

• Antibody Optimization: Developing monoclonal antibodies with higher specificity could improve detection reliability.