At4g18930 Antibody

Introduction to At4g18930 Antibody

The At4g18930 Antibody is a polyclonal rabbit antibody targeting the Arabidopsis thaliana protein encoded by the At4g18930 gene. This protein belongs to the RNA ligase/cyclic nucleotide phosphodiesterase family and is implicated in enzymatic activities such as RNA/DNA processing and cyclic nucleotide metabolism. The antibody is primarily used in immunological assays to study protein expression, localization, and functional interactions in plant biology research .

Structure and Function of At4g18930 Protein

The At4g18930 gene encodes a cyclic phosphodiesterase (EC 3.1.4.-), which hydrolyzes phosphodiester bonds. Biochemical studies confirm its enzymatic activity, including RNA degradation and potential involvement in 2′,3′-cAMP/cGMP synthesis, a pathway linked to plant immune signaling . Key features include:

• Gene Names: At4g18930, F13C5.100, F13C5_100

• Protein Family: RNA ligase/cyclic nucleotide phosphodiesterase

Applications in Research

The antibody is employed in:

1. Protein Detection: Identifying At4g18930 expression in plant tissues via Western blot.

2. Functional Studies: Investigating enzymatic activity in RNA/DNA processing and cAMP/cGMP metabolism.

3. Immune Signaling: Exploring its role in plant pathogen resistance pathways .

Enzymatic Activity and Biochemical Role

Biochemical assays demonstrate that At4g18930 exhibits:

• RNA/DNA Degradation: Hydrolysis of RNA (e.g., Arabidopsis genomic RNA) and plasmid DNA .

• Cyclic Nucleotide Synthesis: Potential involvement in producing 2′,3′-cAMP/cGMP, a secondary messenger linked to immune responses .

Functional Relevance in Plant Immunity

Studies suggest At4g18930 may interact with TIR-domain proteins (e.g., L7 TIR) in:

• Nucleotide Metabolism: Complementing NADase activity to modulate immune signaling.

• Pathogen Defense: Regulating cell death and defense gene activation .

Table 2: At4g18930 Functional Insights