CPN60B1 Antibody

Definition and Target Specificity

CPN60B1 antibody recognizes the Chaperonin 60 subunit beta 1 (CPN60B1), a member of the type I chaperonin family involved in protein folding within chloroplasts. Key details include:

Key Functional Domains

• Subunit Composition:

  • Two α-subunits (Cpn60α1/α2)

  • Four β-subunits (Cpn60β1–β4)

• Protein Interactions: Binds RuBisCO large and small subunits during oligomer assembly .

Cross-Reactivity and Species Specificity

The antibody exhibits broad cross-reactivity across plant species, validated by immunoblotting and immunofluorescence:

Protein Localization Studies

• Detects CPN60B1/B2 in chloroplasts under normal conditions .

• Identifies heat-inducible nuclear translocation of cpn60-related proteins during stress (e.g., heat shock) in animal cells, suggesting conserved stress-response roles .

Stress Response Mechanisms

• In fish cell lines, anti-cpn60 antibodies revealed nuclear accumulation of 57 kDa and 42 kDa proteins post-heat shock, distinct from stress70 family dynamics .

• Implicates CPN60B1 in chaperoning nuclear protein assembly during stress .

Key Research Findings

• Heat Shock Adaptation: Nuclear localization of cpn60 homologs during thermal stress suggests a conserved role in stress adaptation across kingdoms .

• Antibody Specificity: Reacts with cpn60 isoforms but not TCP1 (cytoplasmic chaperonin), confirming target selectivity .

Practical Considerations

• Experimental Use: Ideal for immunoblotting, immunofluorescence, and protein interaction studies in plant systems.

• Limitations: Cross-reactivity with CPN60B2 necessitates isoform-specific validation (e.g., CRISPR mutants) .